ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

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ChromGate® PDA Option 213 Fig. 312 Retention time When the spectrum was extracted from a chromatographic run, the software automatically identifies the retention time of the spectrum and enters the value in this field. When desired, the value in this field may be edited. This field can also be used as a search pre-filter. Comment Enter any descriptive information desired related to the spectrum. This field is used only for display and documentation purposes. Library Notes This field is used to document relevant information about the library as a whole, e.g., documentation about run conditions or general sample information. Fields in existing library entries may be edited by selecting the fields with the cursor. Right-click on a row in the library table to display a pop-up menu used to cut, paste, copy, and insert and delete lines from the library as necessary. Spectrum Information Select the retention time at which you wish to use the spectrum, or the name of the peak whose spectrum is to be used. How to Collect Spectra for a Library 1. Perform an acquisition using a sample with known components or standards to be placed in the library. 2. Perform an analysis using the appropriate integration parameters. (Ideally, Spectral Background Correction would be checked in the Method/PDA Options/Spectrum tab.) 3. Using the contour plot in the Mixed view window, drag the vertical cursor to the apex of a peak to display the corresponding spectrum in the spectrum view. See also Extracting Spectra and Chromatograms from the Contour. 4. Right-click within the spectrum view to display the Spectrum pop-up menu, select Utilities/Save Trace and enter a file name in the dialog box. Repeat this procedure for each spectrum to be added to the library. Files are saved automatically with the .spc extension. To add the 1st or 2nd Derivative of a spectrum to the library, select the appropriate filter in the Method/PDA Options/Spectrum tab and repeat steps 3 and 4. How to Add Spectra to a Library 1. When all of the spectra are saved as .spc files, select File/Library/New to display the Library Definition dialog box and create a new library. Alternatively, select File/Library/Open to add spectra to an existing library. 2. Click in the Spectrum File cell of row 1 to display the Open dialog box. Double-click on the appropriate .spc file in the list box. The .spc
214 ChromGate® PDA Option Fig. 313 file name is entered into that cell and the associated spectrum is simultaneously displayed. A component name and comment may be entered in the appropriate columns. Repeat this procedure with subsequent rows in the table until all spectrum files are entered into the library. 3. Select File /Library /Save As and enter a name for the library. The .lib extension is automatically appended. A new easy way to add spectra to an opened library is to select the option Add Spectrum to Spectrum Library… from the Actions… menu. Move the cursor in the chromatogram view to the appropriate position. The corresponding time is displayed in the dialog box. Simply click on Add to save the spectrum in the library. Library Search How to Perform a <strong>Manual</strong> Library Search 1. In the Contour map of the Mixed View window, drag the vertical cursor to the apex of the peak of interest to display the corresponding spectrum in the spectrum pane. 2. Click on the Actions/Search library… Click either Method to use the library parameters from the method, or Quick to enable you to modify the search parameters. Before you do the search, make sure you have either selected a library in your method, or have opened a library to do a quick search. Click Search Now to display the Library Search Results window, showing the three closest matches in the specified library. When appropriate, click on the >> or
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Software ChromGate ® Chromatograph
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4 CONTENTS Configuration - Autosamp
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6 CONTENTS Peak Table .............
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8 Installation Guide ChromGate® 3.
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10 Installation Guide ChromGate® 3
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12 Installation Guide ChromGate® 3
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14 Short guide ChromGate®, KNAUER
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32 Setup and Control of Knauer HPLC
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84 Creating an Instrument Control M
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100 Creating an Instrument Control
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Page 161 and 162: 162 Creating an Instrument Control
Page 169 and 170: 170 Knauer Instrument Control Metho
Page 185 and 186: 186 ChromGate® System Suitability
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Page 189 and 190: 190 ChromGate® PDA Option Fig. 283
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Page 221 and 222: 222 PDA Analysis and Calculations c
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Page 229 and 230: 230 ChromGate® Preparative Option
Page 257 and 258: 258 SEC Option Universal Calibratio
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264 SEC Option Fig. 373 SEC peak an
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266 SEC Option Fig. 376 Fig. 377 Yo
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268 SEC Option Fig. 379 Enable Anal
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270 SEC Option Export settings Clic
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272 SEC Option All samples analyzed
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274 SEC Option Horizontal Baseline
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276 SEC Option Intrinsic Viscosity
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278 SEC Option Fig. 383 information
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280 SEC Option Fig. 386 Fig. 387 Se
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282 SEC Option single variable sear
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284 Typical Wiring Schemes Typical
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286 Typical Wiring Schemes Fig. 390
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288 Typical Wiring Schemes Fig. 392
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290 Fig. 394 Pump K-1001 / Autosamp
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292 Index Device ID Autosampler 68,
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294 Index R Rack configuration 243
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