ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

122 Creating an Instrument Control Method
Fig. 175 Autosampler Triathlon setup window
Standard Injection
In the Standard Injection section you can enter parameters for the
standard actions that can be performed with the autosampler.
Injection Method
Click one of the Flushed, Partial or µl Pick-up fields to select the
injection method.
The maximum programmable injection volumes are:
Flushed loop: not programmable, is equal to the loop volume but
needs more sample to fill the loop completely
(3 x loop volume for loop volumes < 100 µl and 2 x loop
volume for loop volumes > 100 µl)
Partial loop fill: 50 % of the programmed loop volume
µl Pick-up: Injection volume = (loop volume – 3 x needle volume) / 2
Syringe Speed and Scale Factor
The aspirating speed of the syringe used in injection methods can be
adapted depending on the viscosity of the samples (the choices are Low,
Normal, and High). Alternatively, syringe speeds can be reduced by
entering a scale factor (1 to 10). The syringe speed will be the scale
factor multiplied by the syringe speed. The syringe speed during the
washing or the rinsing procedures is not affected by this setting.
Air Segment
Check the Air Segment box to enable the use of an air segment between
the sample and the wash solvent to reduce the amount of flush volume.
Headspace Pressure
Check the Headspace Pressure box to enable the use of headspace
pressure in combination with the syringe to transport sample to the loop.
The compressor will always be used during a wash procedure.
The accuracy and reproducibility of the autosampler may decrease
if headspace pressure is switched off.
Needle Wash
Check the Needle Wash box to enable a wash procedure.
Flush Volume
Enter the amount of sample in µl taken from a vial to preflush the needle
before the loop is filled with the sample. The default value is 30 µl for an
installed tube volume of 15 µl.