ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

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ChromGate® PDA Option 207 Fig. 303 By default, the Similarity View will use the current method settings. To override the method settings, click the Override method settings box, then enter the values you wish to use. Wavelength range Specify the wavelength range over which the purity calculations will be performed, for example, from 200 nm to 400 nm. Wavelength Step Specify the wavelength spacing (in nm) to be used when purity calculations are performed. Purity threshold The comparison of two spectra gives a Similarity Index (SI), the closer to 1.00 number is the more similar the spectra are. The purity threshold is used to eliminate spectra that do not match. If the SI is greater than the purity threshold, the spectra will be considered pure. In general, a spectra with an SI greater than 0.9900 would have a high probability of being the same as the apex spectra. A SI greater than 0.9000 but less than 0.9900 shows some similarity but would need to be evaluated with care and caution. SI less than 0.9000 should be considered non-similar. Absorbance threshold This value represents the percentage of peak height that spectra will include in purity calculation. Spectra in sections of the peak that do not exceed this threshold will not be included in the purity calculation. This provides a method of eliminating spectra where the concentration of the compound is so low that the solvent spectrum interferes. Background compensation Checking this box will cause spectra to be corrected for background using the peak baseline prior to being used in the calculation of purity. Multi-Chromatogram View This displays multiple chromatographic plots of absorbance versus time, each at a different wavelength. Maximize the PDA display and select View /PDA Views /Multi-Chromatogram (or use the PDA toolbar/Views) to display all of the chromatograms in their respective channels which were specified in the Method / PDA Options / Multi-Chromatogram tab. Right-click within the window to display the pop-up context menu.
208 ChromGate® PDA Option Fig. 304 Spectrum View Fig. 305 Fig. 306 This view displays the spectrum associated with a time on the chromatogram. The time of the spectrum to be displayed can be changed from Mixed View by sliding the spectrum selection box (on the x-axis). Spectrum Properties Select this menu item to access the trace properties dialog. This enables you to add another trace to the view, or change scaling. It also lets you selectively remove overlaid traces from the view. Up to 3 wavelength maxima and –minima can be displayed. Use the Annotations… command from the right mouse click menu in the Spectrum view and select Lambda Max and/or Lambda Min.
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Software ChromGate ® Chromatograph
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4 CONTENTS Configuration - Autosamp
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6 CONTENTS Peak Table .............
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8 Installation Guide ChromGate® 3.
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10 Installation Guide ChromGate® 3
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12 Installation Guide ChromGate® 3
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14 Short guide ChromGate®, KNAUER
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32 Setup and Control of Knauer HPLC
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84 Creating an Instrument Control M
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100 Creating an Instrument Control
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Page 155 and 156: 156 Creating an Instrument Control
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Page 189 and 190: 190 ChromGate® PDA Option Fig. 283
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258 SEC Option Universal Calibratio
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260 SEC Option Fig. 369 Acquisition
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262 SEC Option Amount Values In thi
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264 SEC Option Fig. 373 SEC peak an
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266 SEC Option Fig. 376 Fig. 377 Yo
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268 SEC Option Fig. 379 Enable Anal
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270 SEC Option Export settings Clic
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272 SEC Option All samples analyzed
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274 SEC Option Horizontal Baseline
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276 SEC Option Intrinsic Viscosity
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278 SEC Option Fig. 383 information
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280 SEC Option Fig. 386 Fig. 387 Se
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282 SEC Option single variable sear
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284 Typical Wiring Schemes Typical
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286 Typical Wiring Schemes Fig. 390
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288 Typical Wiring Schemes Fig. 392
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290 Fig. 394 Pump K-1001 / Autosamp
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292 Index Device ID Autosampler 68,
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294 Index R Rack configuration 243
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ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

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