ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

68 Setup and Control of Knauer HPLC Systems
Device ID
Select the device identifier for the autosampler from the drop-down list.
This must match the settings of the autosampler being used. To check
the device identifier for the autosampler Knauer Optimas, press the
SYSTEM, MENU and then SERIAL buttons on the autosampler
keyboard. The default identifier is 60.
Serial Port
Select the number for the communication port on your PC where the
autosampler is connected. On the autosamplers rear panel use the port
S2 IN, to connect the autosampler with the computer. It is recommended
to switch all DIP switches for the port to OFF position for a proper
communication.
Loop Volume
Enter the volume of the installed loop in µl. If the Prep mode is enabled,
the loop volume is fixed on 10000 µl.
Tubing Volume
Type in volume of the installed needle and tubing in µl. The valid range is
0 - 999 µl. The volume is noted on a label on the tube.
Syringe Volume
Select the volume of installed syringe from the drop-down list. The
choices are 250 µl, 1000 µl, and 2500 µl. If the 2500 µl syringe is
selected, automatically the Prep mode check box is enabled. In the Prep
mode the values for the syringe (2500 µl) and loop (10000 µl) and the 24
vial tray type are fixed.
Options (Tray cooling / Prep mode)
Check the appropriate boxes if you want to control the tray cooling or
enable the Prep mode. The desired tray temperature can be entered
later as part of the instrument setup. In the Prep mode the values for
syringe (2500 µl) and loop (10000 µl) and the 24 vial tray type are fixed.
The tubing volume is set to 45 µl. Please check the correct volume of the
tube between the needle and the injection valve on the tube’s label.
Tray...
Click the Tray button to set up the autosampler tray: