ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

120 Creating an Instrument Control Method
will be made per run. Please make sure that you have enough samples
for the entered number of injection.
Sample Vial
Select the vials the stacked injection should be made from. Select Same
to injection from the same vial as the previous injection, Next to inject
from the vial number from the previous injection or Custom to
select free the vial number.
Injection Volume (µl)
Select the desired injection volume. Note that the volume depend from
the selected injection mode of the autosampler instrument setup.
Sample Preparation Time (min)
Define the time before the injection time; the autosampler should start
with the sample/injection preparation. Please make sure, that the given
time is sufficient to prepare the injection before the injection time arrives,
otherwise the injection may be delayed. If the autosampler is ready to
inject, it will wait until the programmed time for the stacked injection. If the
autosampler needs a longer time than programmed, it will inject
immediately after the injection preparation is finished. In this case the
next stacked injection will be delayed by this “extra time”.
Time between Injections (min)
Enter the time between the injections.
Do not wash needle between injections from same vial
Enable this option if you wish to prohibit the needle wash after each
injection, programmed in the autosamplers instrument setup, for the
stacked injections from the same vial.
If you close the Stacked Injections window with Apply, the selected
settings will be copied into the Stacked injections table. Any existing
entries from previous programming will be deleted.
If you make a right mouse click on the table, you can select
- Fill Down, to fill the table with the same settings as in the selected line
- Insert Line, to add a new line
- Delete Line, to delete the selected line
- Delete Lines, to delete all selected lines
- Fill Table…, will open the Stacked injection setup again. The new table
will overwrite the existing one. If you select the command Fill table to
open the setup dialog for the stacked injection table, the window will
show default values for all parameters, beside the sample preparation
time.
Click on OK to close the Stacked Injections table window; the
autosampler S 3950 instrument setup window will be shown.
In the Aux Traces tab a trace “Stacked Inj. [AS 3950] can be enabled.
The trace will show the start of the sample preparation and the stacked
injection.