ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

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ChromGate® System Suitability Setup 185 ChromGate ® System Suitability Setup Fig. 278 Before ChromGate ® can make System Suitability calculations, you must enter the required acceptance ranges for the peaks of interest. This is done in using the Method – System Suitability command. 1. Click on the Method – System Suitability command to view the System Suitability Setup dialog. 2. Select the first peak to be used for calculations by highlighting it with the mouse in the Compounds list. 3. In the adjacent spreadsheet, click on the first field in the Parameter column. A drop-down list of available parameters is displayed. Select a parameter from the list. If you do not wish to perform a suitability test on any given peak, simply leave the Parameter fields blank. Similarly, if you do not wish to have one of the test criteria used (for example, %RSD), leave it blank. Some parameters have a choice of calculation methods. These parameters have the calculation method displayed after the parameters in parenthesis (e.g. Plates/Meter (JP) indicates Plates/Meter calculated using the Japanese Pharmacopeia calculation). For details on these calculations, see the equations section. 4. For each parameter selected, enter a minimum value (Min), maximum value (Max), and maximum allowed percent relative standard deviation (%RSD). 5. If you want a noise test to be performed, click on the lower spreadsheet in the column labeled Test. Select one of the noise calculations Noise (rms)/ASTM Noise (rms), or 6-sigma Noise (rms) from the drop-down list. If you select this box, you must enter a Start and Stop time for the test, and the Threshold value to determine acceptable limit. The RMS noise value for the portion of chromatogram between the Start and Stop times will be calculated and compared to the Threshold value to determine whether the test Passed or Failed. Note that the times you enter for the noise test should be representative of a baseline area of your chromatogram where not peaks elute. Keep in mind, that the noise may depend on the detector type, wave length, flow rate, solvent and other parameters used in the test.
186 ChromGate® System Suitability Setup Copy & Paste 6. If you want a drift test to be performed, click on the lower spreadsheet in the column labeled Test. Select Drift (uV/min) from the drop-down list. If you select this box, you must enter a Start and Stop time for the test, and a Threshold value (in μV/Min) for the acceptable limit. 7. When you have completed the System Suitability Setup, close the box. After highlighting sections of the spreadsheet that you wish to copy, push: Ctrl C- to copy the highlighted section of the spreadsheet to the clipboard. Shift + Insert- to paste the clipboard item to where the cell is highlighted. Shift + Arrows- highlights the spreadsheet cells, and can move them. Suitability Calculation Selection Fig. 279 System Suitability calculations require information about your chromatography column. These parameters are entered in the Method/<strong>Advanced</strong>/Performance tab dialog box. 1. Click on Method/<strong>Advanced</strong>/Performance tab. Enter values for the column parameters shown, required for calculation of performance options.
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Software ChromGate ® Chromatograph
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4 CONTENTS Configuration - Autosamp
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6 CONTENTS Peak Table .............
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8 Installation Guide ChromGate® 3.
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10 Installation Guide ChromGate® 3
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12 Installation Guide ChromGate® 3
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14 Short guide ChromGate®, KNAUER
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32 Setup and Control of Knauer HPLC
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84 Creating an Instrument Control M
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100 Creating an Instrument Control
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Page 133 and 134: 134 Creating an Instrument Control
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236 ChromGate® Preparative Option
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258 SEC Option Universal Calibratio
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260 SEC Option Fig. 369 Acquisition
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262 SEC Option Amount Values In thi
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264 SEC Option Fig. 373 SEC peak an
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266 SEC Option Fig. 376 Fig. 377 Yo
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268 SEC Option Fig. 379 Enable Anal
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270 SEC Option Export settings Clic
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272 SEC Option All samples analyzed
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274 SEC Option Horizontal Baseline
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276 SEC Option Intrinsic Viscosity
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278 SEC Option Fig. 383 information
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280 SEC Option Fig. 386 Fig. 387 Se
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282 SEC Option single variable sear
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284 Typical Wiring Schemes Typical
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286 Typical Wiring Schemes Fig. 390
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288 Typical Wiring Schemes Fig. 392
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290 Fig. 394 Pump K-1001 / Autosamp
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292 Index Device ID Autosampler 68,
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294 Index R Rack configuration 243
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