ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

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ChromGate® PDA Option 205 Fig. 299 Fig. 300 Fig. 301 Peak Purity Plot The Total Peak Purity view displays the purity profile for a chromatogram extracted from the 3D data. The view displays purity information for the Max Plot chromatogram that is displayed in the Chromatogram pane of the Mixed View. The pane will be blank until the data has been analyzed, and a peak has been selected for the purity calculation. Select Peak for Purity Similarity Display To select the reference spectrum for the purity calculation, make sure the data has been analyzed. (If you are not sure, click the analyze button on the toolbar.) Then, click Actions/Select Peak from the Mixed View toolbar. A dialog will appear that instructs you to select a peak by holding down the Ctrl button and then clicking on a peak from the Max Plot pane. The retention time of the reference spectrum is displayed along with a value indicating peak purity.
206 ChromGate® PDA Option Fig. 302 You can continue to select or change the peak. When you are finished, click the Close button. Purity View Properties To set up the properties for the Purity View, do a right mouse click, and select Properties. By default, the Similarity View will use the current method settings. To override the method settings, click the Override method settings box, then enter the values you wish to use. Wavelength range Specify the wavelength range over which the purity calculations will be performed, for example, from 200 nm to 400 nm. Wavelength Step Specify the wavelength spacing (in nm) to be used when purity calculations are performed. Purity threshold The comparison of two spectra gives a Similarity Index (SI), the closer to 1.00 number is the more similar the spectra are. The purity threshold is used to eliminate spectra that do not match. If the SI is greater than the purity threshold, the spectra will be considered pure. In general, a spectra with an SI greater than 0.9900 would have a high probability of being the same as the apex spectra. A SI greater than 0.9000 but less than 0.9900 shows some similarity but would need to be evaluated with care and caution. SI less than 0.9000 should be considered non-similar. Absorbance threshold This value represents the percentage of peak height that spectra will include in purity calculation. Spectra in sections of the peak that do not exceed this threshold will not be included in the purity calculation. This provides a method of eliminating spectra where the concentration of the compound is so low that the solvent spectrum interferes. Background compensation Checking this box will cause spectra to be corrected for background using the peak baseline prior to being used in the calculation of purity. Similarity View Properties To set up the properties for the Similarity View, do a right mouse click, and select Properties.
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Software ChromGate ® Chromatograph
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4 CONTENTS Configuration - Autosamp
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6 CONTENTS Peak Table .............
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8 Installation Guide ChromGate® 3.
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10 Installation Guide ChromGate® 3
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12 Installation Guide ChromGate® 3
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14 Short guide ChromGate®, KNAUER
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32 Setup and Control of Knauer HPLC
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84 Creating an Instrument Control M
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100 Creating an Instrument Control
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Page 153 and 154: 154 Creating an Instrument Control
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Page 229 and 230: 230 ChromGate® Preparative Option
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256 ChromGate® Preparative Option
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258 SEC Option Universal Calibratio
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260 SEC Option Fig. 369 Acquisition
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262 SEC Option Amount Values In thi
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264 SEC Option Fig. 373 SEC peak an
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266 SEC Option Fig. 376 Fig. 377 Yo
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268 SEC Option Fig. 379 Enable Anal
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270 SEC Option Export settings Clic
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272 SEC Option All samples analyzed
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274 SEC Option Horizontal Baseline
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276 SEC Option Intrinsic Viscosity
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278 SEC Option Fig. 383 information
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280 SEC Option Fig. 386 Fig. 387 Se
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282 SEC Option single variable sear
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284 Typical Wiring Schemes Typical
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286 Typical Wiring Schemes Fig. 390
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288 Typical Wiring Schemes Fig. 392
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290 Fig. 394 Pump K-1001 / Autosamp
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292 Index Device ID Autosampler 68,
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294 Index R Rack configuration 243
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ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

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