ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

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ChromGate® PDA Option 221 Peak Table 3 Point Purity Checking this indicates that 3 point peak purity should be printed to the right of the spectrum graph. Similarity Checking this indicates that peak apex similarity to the reference spectrum should be printed to the right of the spectrum graph. The use of this value requires that a reference spectrum be included in the peak table. See the section on Analysis and Calculations for details. Spectral Display The controls in this group specify the content and labeling of the spectrum graphs. Show Lambda Maxima Checking this box will annotate each spectrum graph with the 3 largest lambda max absorbance. Show Lambda Minima Checking this box will annotate each spectrum graph with the 3 largest lambda minima absorbance. Background Correction Checking this will cause each apex spectrum to be corrected for background using the chromatographic baseline prior to being used elsewhere in analysis. Refer to the PDA Analysis and Calculations section for details on the formula used. Filtering Type This allows the user to specify a mathematical filtering function to be performed on all spectra extracted from the 3D data during analysis. Refer to the PDA Analysis and Calculations section for details on the formula used for each filter. Graph Height This specifies the relative height of the spectrum graph. A value of 100% corresponds to a standard sized graph. A larger value may be selected to provide more a detailed plot. Graph Properties Pressing this button displays the graph properties dialog as found on the standard Spectrum graph. When using the PDA option, certain columns will be added to the peak table that enable you to analyze peaks from the PDA detector. Detection Select the basis for the identification of the peak. If you choose Ret Time, only the retention time will be used for identification of the peak. If you choose Ret Time with Spectral Confirm, the Similarity of the peak’s spectrum to that of a designated reference spectrum will be used in addition to the retention time as the basis of peak identification. Spectrum If you want Similarity to be used as a basis for peak identification, then click on the arrow to the right of this field to specify the stored reference spectrum to be used for comparison. During identification, this reference spectrum is compared to the peak apex spectrum and a similarity index is
222 PDA Analysis and Calculations computed. A peak is considered identified if this calculated similarity index is at least the value specified in the Similarity column of the peak table. If Similarity is not specified as a basis for peak identification, then this field is ignored. Similarity If Similarity is specified as a basis for peak identification, then this field specifies required minimum similarity index for a peak to be considered identified. During identification, the reference spectrum (see previous section) is compared to the peak apex spectrum and a similarity index is computed. A peak is considered identified if the calculated similarity index is at least the value in this field. If Similarity is not specified as a basis for peak identification, then this field is ignored. Analysis Channel Specify which Diode Array wavelength channel is to be used for analysis of the peak. The choices will be those specified in Instrument Setup/DAD. PDA Analysis and Calculations This section describes the various types of calculations that are related to PDA data and how the data is analyzed. In order to maintain accuracy during the application of multiple operations, all calculations are performed using double-precision floating-point numbers. Chromatograms Extracted from the 3D Data Two types of chromatograms may be extracted from the 3D data: � Multi-Chromatogram Channels: One or more chromatograms defined on the Multi-Chromatogram tab of PDA Options � Working Chromatogram: A single chromatogram extracted from the Contour plot on the Mixed View display. (This chromatogram is displayed in the Chromatogram View) Specific Analysis-related capabilities are for these chromatograms are detailed below. Multi-Chromatogram Channels The following apply to multi-chromatogram channels: � In the Channel Selection drop-down list, each multi-chromatogram channel has a unique identifier that includes the wavelength and bandwidth of the channel. � Each Multi-Chromatogram Channel has a separate Integration Events Table, <strong>Manual</strong> Integration Table, Export Table and Performance Table associated with it. � All Multi-Chromatogram Channels share a common peak and group table. Within the peak and group tables, any channels may be selected as the analysis channel for quantitative information. � When an analysis is performed, all multi-chromatogram channels are automatically analyzed.
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Software ChromGate ® Chromatograph
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4 CONTENTS Configuration - Autosamp
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6 CONTENTS Peak Table .............
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8 Installation Guide ChromGate® 3.
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10 Installation Guide ChromGate® 3
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12 Installation Guide ChromGate® 3
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14 Short guide ChromGate®, KNAUER
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32 Setup and Control of Knauer HPLC
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84 Creating an Instrument Control M
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100 Creating an Instrument Control
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Page 169 and 170: 170 Knauer Instrument Control Metho
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Page 229 and 230: 230 ChromGate® Preparative Option
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Page 267 and 268: 268 SEC Option Fig. 379 Enable Anal
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272 SEC Option All samples analyzed
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274 SEC Option Horizontal Baseline
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276 SEC Option Intrinsic Viscosity
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278 SEC Option Fig. 383 information
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280 SEC Option Fig. 386 Fig. 387 Se
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282 SEC Option single variable sear
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284 Typical Wiring Schemes Typical
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286 Typical Wiring Schemes Fig. 390
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288 Typical Wiring Schemes Fig. 392
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290 Fig. 394 Pump K-1001 / Autosamp
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292 Index Device ID Autosampler 68,
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294 Index R Rack configuration 243
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