ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

118 Creating an Instrument Control Method
Headspace Pressure
Check the Headspace Pressure box to enable the use of headspace
pressure in combination with the syringe to transport sample to the loop.
The compressor will always be used during a wash procedure.
The accuracy and reproducibility of the autosampler may decrease
if headspace pressure is switched off.
Needle Wash
Check the Needle Wash box to enable a wash procedure.
Temperature control
If your autosampler 3950 is equipped with a tray cooling facility, this
option can be checked and the desired temperature (4 – 22 °C) can be
entered.
Wait with tolerance (±°C)
The system becomes ready only if the actual tray temperature is inside
the set range.
Wait time (min)
The waiting time is the equilibrating time in minutes after the tray temperature
reached the temperature set range before the injection takes
place.
Mix Methods...
Click the Mix Methods button to create a program of sample preparation.
The Mix Methods set up dialog will appear. It is the same dialog as
already described for the AS 3900, starting with Fig. 165 on page 115.
Please note, that for the autosampler 3950 for all tray types except the
83+3 vials tray no transport vial for the µl pick-up can be defined. The
transport liquid will be escaped from the wash solution. If the 84+3 vials
tray is selected, the vials 85, 86 or 87 can be selected as a transport vial.
The transport plug is 2.5 x needle volume (needle volume = tubing
volume, please refer to the autosampler configuration chapter). The
autosampler itself calculates the liquid level in the transport vials. It will be
reset to 8000 µl every time if the autosampler is switched off and on.
Additionally the level can be reset from the autosamplers direct control
tab. The level cannot be read-out from the software. If the autosampler
calculates, that the vial is empty, the method/sequence will stopped.
Dependent from the transport liquid level, the autosampler will adapt the
needle immersion depth. Therefore it is important to fill the transport vials
whenever the autosampler was switch off or the transport liquid level was
reset manually. If a vial is selected as the transport vial, the configuration
of the vial as a Reagent A or B vial will be ignored.
Stacked injections...
The option Stacked injections allow to define additional injections during a
run. Such injections can only be programmed either for the autosampler
3950 or the injection module (please refer the FRC section, chapter
“Stacked Injections” of this manual). Click on the Stacked injections –
button to open the Stacked Injections table dialog.
Enable the check box “Use stacked injection”.