ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

276 SEC Option
Intrinsic Viscosity
If you have selected Intrinsic Viscosity for the Use Constants field, enter
the value here. Otherwise this field is not active.
Time Slice
Select a time slice width (in seconds) for the calculations. The slice width
will determine the frequency of Molecular Mass fractions reported. If a
Slice Report is generated, it will be based on the slice width you select
here. The software will force this number to be an integral of the data
collection rate during the analysis.
Baseline Start and Stop Times
The baseline start and stop times (in minutes) can be entered manually
or selected graphically using the SEC buttons on the chromatogram
toolbar.
Results Range Start and Stop
One or more ranges of the chromatogram must be defined to be used in
calculation and reporting of the Molecular Mass distribution. Each range
is determined by the results range start and stop values. The times (in
minutes) can be entered manually or graphically using a stored
chromatogram.
Horizontal Baseline
Select this button if you wish the SEC baseline to be drawn as a
horizontal line forward from the Baseline Start Time to the Stop Time.
The horizontal baseline stops when it crosses the data trace. A threshold
value in microvolt must be specified to ignore any detector baseline noise
in the trace that would cause the horizontal projection to stop when it
encounters the noise in the trace. If the Horizontal Baseline reaches the
end of the detected peak before the programmed Stop Time, the range
will stop where the Horizontal Baseline crosses the data trace.
Calibration Data
Enter values for Mn and Mx in the fields provided or k and a, then click the
Calibrate button. The system will calculate k and a or Mn and Mx values
to be used for the calibration. You must have completed the Molecular
Mass table before entering the calibration data.
Reference Peak Time (min)
Select this box if you are using a retention time reference peak to adjust
for changes in flow rate. All elution times/volumes used in calculations
will be offset by the time difference between the value entered here and
the time of the reference peak in the sample. Enter the expected
retention time for the reference peak, in minutes.
Reference Window (%)
If you are using a reference peak, enter a value for the % window for the
reference peak. A peak falling within +/- this % of the expected retention
time for the reference peak will be considered the reference peak. (If two
peaks fall within the window, the peak closest to the center will be chosen
as the retention time reference.)