ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

152 Creating an Instrument Control Method
results in an increased signal to noise ratio because the multiple
measurements cause the random noise to be cancelled out. That is, if the
noise is truly random, more measurements will decrease the noise
because for each positive excursion in the noise will have a good
likelihood of being cancelled by a negative excursion. But the signal,
since it is not random, will remain the same over multiple measurements.
Bandwidth is a tradeoff, with a high value decreasing the noise but also
decreasing the possible resolution which can be measured. 5 nm is the
default value, but if your peaks have substantially wider UV spectra you
can increase the bandwidth value in ChromGate ® to obtain a lower noise
level.
Mode
Select the signal mode for your measurements from the drop-down list.
The choices are Absorption and Intensity.
Open/Close Shutter
A click on this button will open/close the shutter. The label will be
changed accordingly.
Filter On/Off (S 2600 only)
You can click on this button depending on whether or not you are using
an edge filter.
Dark Signal
Click the button to measure the dark current. This operation is enabled
only for devices equipped with a shutter.
Set Reference
Click the button to measure a spectrum to be used as a reference
spectrum.
Spectrum
Click the button to measure an absorption or intensity spectrum,
depending on the signal mode selected for the measurement. The
measured spectrum will be shown in the spectrum view.
Instrument Status – Fluorescence Detector RF-10Axl / RF-20A
Fig. 226 Detector Status tab, Fluorescence Detector RF-10Axl
Status
The status HOLD will be displayed when no program is running;
otherwise the Run Status displays a run time in minutes elapsed from the
start of the run.
The actual excitation wavelength, emission wavelength, signal value,
sensitivity, gain, and the selected output mode will be displayed.