ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

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Creating an Instrument Control Method 115 CAUT ION The Stop Events at Time allows setting the end time of the timed events. If the Stop Events at Time box is not checked, the end time is equal to the analysis time (the run time). The maximal Stop events time is 9 h 59 min 59 sec. If the END time exceeds the programmed run time, this END time overrules the analysis time for the autosampler. The next injection will be started as soon as the timed events program has finished. Mix Methods... Click the Mix Methods button to create a program of sample preparation. The Mix Methods set up dialog will appear. Fig. 165 Autosampler 3900/3950 Mix Methods Clicking the New button first the method type drop-down list becomes active. The mix method can contain a maximum of 15 programmable lines including the END OF MIX command. Click the New button to add one more line to the programmed methods. Select the Method Type from the drop-down list. The choices are Add, Mix and Wait. Fig. 166 Autosampler 3900/3950 Mix Step Add Specify the Amount of a liquid in µl (max = the syringe volume) to be aspirated from a source (choices are Sample, Reagent A, Reagent B and Wash) and dispensed to a target (choices are Sample and Destination). The aspirate and dispense speed depends on the selected syringe and syringe speed. The reagents and transport solvent are located at the following positions on the tray: Transport solvent: vial 85 Reagent A: vial 86 Reagent B: vial 87
116 Creating an Instrument Control Method Fig. 167 Autosampler 3900/3950 Mix Step Mix According the number of Mix Times (1-9 times) the programmed Amount (µl, max = the syringe volume) will be aspirated and dispensed. If no destination vial is used the mixing step is performed in the sample vial. Fig. 168 Autosampler 3900/3950 Mix Step Wait Wait a programmed period of time x:xx:xx (0:00:00 – 9:59:59) before continuing with the next step (reaction time). Click the Commit button to complete the operation. Click the Delete button to delete the selected step. Instrument Setup – Autosampler 3950 (Alias) Fig. 169 Autosampler 3950 setup window
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Software ChromGate ® Chromatograph
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4 CONTENTS Configuration - Autosamp
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6 CONTENTS Peak Table .............
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8 Installation Guide ChromGate® 3.
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10 Installation Guide ChromGate® 3
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12 Installation Guide ChromGate® 3
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14 Short guide ChromGate®, KNAUER
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32 Setup and Control of Knauer HPLC
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166 Creating an Instrument Control
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168 Creating an Instrument Control
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170 Knauer Instrument Control Metho
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186 ChromGate® System Suitability
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188 ChromGate® System Suitability
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190 ChromGate® PDA Option Fig. 283
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192 ChromGate® PDA Option calculat
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194 ChromGate® PDA Option (to the
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196 ChromGate® PDA Option PDA View
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216 ChromGate® PDA Option Note tha
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222 PDA Analysis and Calculations c
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224 PDA Analysis and Calculations s
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226 PDA Analysis and Calculations u
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228 PDA Analysis and Calculations 3
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230 ChromGate® Preparative Option
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258 SEC Option Universal Calibratio
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260 SEC Option Fig. 369 Acquisition
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262 SEC Option Amount Values In thi
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264 SEC Option Fig. 373 SEC peak an
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266 SEC Option Fig. 376 Fig. 377 Yo
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268 SEC Option Fig. 379 Enable Anal
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270 SEC Option Export settings Clic
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272 SEC Option All samples analyzed
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274 SEC Option Horizontal Baseline
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276 SEC Option Intrinsic Viscosity
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278 SEC Option Fig. 383 information
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280 SEC Option Fig. 386 Fig. 387 Se
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282 SEC Option single variable sear
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284 Typical Wiring Schemes Typical
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286 Typical Wiring Schemes Fig. 390
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288 Typical Wiring Schemes Fig. 392
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290 Fig. 394 Pump K-1001 / Autosamp
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292 Index Device ID Autosampler 68,
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294 Index R Rack configuration 243
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ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

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