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ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

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88 Creating an Instrument Control Method<br />

In the first row (at time 0.00 min), enter the initial flow (in ml/min), solvent<br />

composition (in %), and digital events. Then for (time in minutes), you can<br />

define the pump parameters. For KNET pumps up to 50 steps are<br />

allowed with an accepted minimum time difference of 0.02 minutes<br />

between two lines.<br />

The complete program is transferred or “uploaded” to the pump when you<br />

start a run.<br />

All time programs are displayed in a spreadsheet similar to the one<br />

shown below. Although the information in the fields will vary, the<br />

spreadsheets always support certain basic features.<br />

Fig. 130 Editing tools for the time (gradient) program<br />

Each row is assigned a program line number and time in minutes,<br />

followed by columns for information for each time in the program. Rows<br />

and field information can be cut, copied, pasted, and cleared. To open<br />

the menu with these commands, right-click anywhere within the<br />

spreadsheet. To select a field, click on that field to highlight<br />

To select a row, click on a number in the # column to highlight the entire<br />

row.<br />

Certain spreadsheet fields will have choices available for you to select.<br />

Fields with selections available will display a combo-box button when the<br />

field or row is selected. Click the button to display the available choices.<br />

Fill Down<br />

This enables you to automatically copy spreadsheet information from one<br />

field or row down through the rest of the spreadsheet.<br />

Insert Line<br />

This command inserts a copy of the line in the spreadsheet below where<br />

the cursor is located.<br />

Delete Line<br />

This command deletes the line in the spreadsheet where the cursor is<br />

located.<br />

Delete Lines<br />

This command deletes the selected lines in the spreadsheet.<br />

The flow and gradient composition profile is displayed in the profile<br />

window area below the spreadsheet.<br />

For the HPG mode, the changes made for a gradient program will be<br />

automatically transferred to the gradient programs of all the pumps<br />

which form this HPG system. This does not affect the event<br />

configuration which can be setup for all pumps independently.

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