ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

262 SEC Option
Amount Values
In this section, you can enter values that affect how the concentrations
are calculated. If you are making a single data acquisition prior to
calibrating your method, simply leave these values at the default level.
Sample Amount
The Sample Amount value is used as a divisor during calculation of
concentrations. It is intended to compensate for differences between
samples due to weighing and when percentages of the total sample are
being calculated rather than the amount detected in an injection.
Internal Standard Amount
For calibration runs, the Internal Standard Amount is taken from the
method Peak Table. For unknown runs, enter the amount of the Internal
Standard in your unknown sample.
Multiplication Factor
Enter a multiplication factor to be used for this run. All analyzed peaks
will be multiplied by this factor.
Calibrate
This box is only used for calibration of partition chromatography samples.
It is not used in SEC runs.
When you have completed the Single Acquisition Run dialog box, click
Start to begin the acquisition. The current data will appear in the
chromatogram window as it is acquired and stored on disk. At the end of
the run, the chromatogram will be analyzed according to the method
parameters, and a report generated if specified. If the sample is not
analyzed at the end of the acquisition, click the Analyze button if you
wish to view the results.
Sample Description
When you click the Description button, you can enter a text description
for the sample that is saved in the data file. The description can be
viewed from the Open File dialog, or when the file is open as the current
data file using the Data/Properties command.
Submit
This button appears when data is currently being acquired using a
sequence or single run. The Submit button allows you to submit a single
acquisition to be run at the completion of the current run. The run is
entered at the end of the Run Queue if you are currently running a
sequence of runs.
Submit Priority
This button will appear when data is currently being acquired using a
sequence. When you click Submit Priority… you can submit the single
run to be executed immediately after the current sequence run record in
the Run Queue. After this sample is finished, the sequence will resume.
If the chromatogram is not integrated at the end of the run, or if you
were expecting a report and none was printed, check the Method/
Properties section of your method to make sure data analysis and
reporting are turned on for this method.