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ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

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128 Creating an Instrument Control Method<br />

Large Injection<br />

Check the Enable large injections box to use this option for injections<br />

larger than 1 ml. This selection sets the standard injection parameters<br />

inactive. It cannot be combined with any user defined injection program.<br />

Instrument Setup – Kontron Autosamplers<br />

Fig. 182 Kontron Autosamplers setup window<br />

Injection type<br />

Needle wash<br />

The standard procedure involves two needle wash cycles after each<br />

injection. Checking this option additionally a needle wash is performed<br />

before each injection.<br />

Injection volume<br />

The injection volume has to be entered for the AS 360, 560, and 565 in<br />

fact as a set of three parameters. The possible values depend on the<br />

configured syringe. They are summarized in the following table:<br />

Syringe Air Gap Sample / Into Loop Sum* )<br />

250 5 to 120 0 to 230 240<br />

500 5 to 240 0 to 470 480<br />

1000 5 to 480 0 to 950 960<br />

2500 5 to 800 0 to 1590 1600<br />

* ) Includes the air gap twice<br />

Air Gap: A defined Air segment is used to separate the sample plug<br />

from the wash liquid in the sampler lines.<br />

Sample: Defines the actual sample volume of sample taken from<br />

the sample vial.<br />

Into Loop: Defines the volume required to transport the sample into<br />

the sample loop.<br />

Aux<br />

Two auxiliary outputs (four for AS 460 and 465) can be programmed. At<br />

the given times a pulse will be sent to the selected output number.<br />

Mix Methods…<br />

This button appears for the AS 460 and 465 only. Click the Mix Methods<br />

button to create a program of sample preparation. The Mix Methods set<br />

up dialog will appear.

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