ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

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SEC Option 263 Defining SEC Baseline Fig. 371 You can use your stored SEC calibration standard chromatogram to establish the SEC baseline for the analysis. With the chromatogram displayed in the chromatogram window, click on the SEC baseline button from the toolbar. Then click the mouse on the chromatogram at the beginning and then the end of the desired SEC baseline. The range you selected will be displayed in the dialog. Click Add to add this baseline range to your SEC method setup. Alternatively, this information can be entered manually in the SEC Setup screen. An SEC baseline is required before SEC analysis can be performed. Defining SEC Result Ranges Fig. 372 You can use your stored SEC calibration standard chromatogram to establish the SEC result range(s) for the analysis. With the chromatogram displayed in the chromatogram window, click on the SEC Range button on the toolbar. Then click the mouse at the beginning and end of each desired result range. Molecular Mass calculations will be made for each range you select. The specified range will be displayed in the dialog box. If the result range falls within a baseline range already entered, that baseline range will be displayed. Click the Add button to enter the designated result range into your SEC setup. Alternatively, this information can be entered manually in the SEC Setup screen. One or more result ranges are required before an SEC analysis can be performed. Define SEC Peaks You can use your stored calibration standard chromatogram(s) to enter peaks and their retention times into your SEC calibration setup. 1. Make sure your calibration standard chromatogram is displayed as the current chromatogram, and that it has been analyzed. (If it has not been analyzed, click on the Analyze button.) 2. Select on the Method/ Define SEC Peaks command, or click the Define SEC Peaks button on the tool bar of the chromatogram window. You will be prompted to click your mouse before the first
264 SEC Option Fig. 373 SEC peak and after the last SEC peak you wish to enter in your SEC setup peak list. Once you have clicked to define the peak region, a box will appear where you select options for the defined peaks. Add Choose this option to add the selected peaks to the current SEC calibration peak table. Existing peaks in the table will be maintained. Replace Choose this option to selectively replace peaks in the current SEC calibration table. The tolerance is used to determine if an existing time should be replaced with a new time. If the tolerance is 0 the numbers must be an exact match. The larger the tolerance percentage is the larger the variance can be between the new value and the existing value and still be considered a match. Average Choose this option to selectively average new times in the current SEC calibration table with existing ones. The tolerance is used to determine if an existing time should be average with the new time. If the tolerance is 0 the numbers must be an exact match. The larger the tolerance percentage is the larger the variance can be between the new value and the existing value and still be considered a match. Only five trial values will be used in the average. If you try to average a sixth time value, the oldest trial time will be thrown out. Tolerance Tolerance % is used to determine “matching” values when using the replace or average options. If the tolerance is 0 the numbers must be an exact match. The larger the tolerance percentage is the larger the variance can be between the new value and the existing value and still be considered a match. Click OK to enter the defined peaks into the SEC Setup table. Annotating SEC Chromatograms When you view an SEC chromatogram in the chromatogram window, there are a number of annotation options available to you that are specific to SEC samples. These are available for you to set when you do a righthand mouse click in the chromatogram window, and select Annotations.
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Software ChromGate ® Chromatograph
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4 CONTENTS Configuration - Autosamp
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6 CONTENTS Peak Table .............
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8 Installation Guide ChromGate® 3.
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10 Installation Guide ChromGate® 3
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12 Installation Guide ChromGate® 3
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14 Short guide ChromGate®, KNAUER
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32 Setup and Control of Knauer HPLC
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84 Creating an Instrument Control M
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100 Creating an Instrument Control
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170 Knauer Instrument Control Metho
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186 ChromGate® System Suitability
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188 ChromGate® System Suitability
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190 ChromGate® PDA Option Fig. 283
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192 ChromGate® PDA Option calculat
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194 ChromGate® PDA Option (to the
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196 ChromGate® PDA Option PDA View
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Page 211 and 212: 212 ChromGate® PDA Option Fig. 310
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Page 221 and 222: 222 PDA Analysis and Calculations c
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Page 229 and 230: 230 ChromGate® Preparative Option
Page 257 and 258: 258 SEC Option Universal Calibratio
Page 259 and 260: 260 SEC Option Fig. 369 Acquisition
Page 261: 262 SEC Option Amount Values In thi
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Page 267 and 268: 268 SEC Option Fig. 379 Enable Anal
Page 269 and 270: 270 SEC Option Export settings Clic
Page 271 and 272: 272 SEC Option All samples analyzed
Page 273 and 274: 274 SEC Option Horizontal Baseline
Page 275 and 276: 276 SEC Option Intrinsic Viscosity
Page 277 and 278: 278 SEC Option Fig. 383 information
Page 279 and 280: 280 SEC Option Fig. 386 Fig. 387 Se
Page 281 and 282: 282 SEC Option single variable sear
Page 283 and 284: 284 Typical Wiring Schemes Typical
Page 285 and 286: 286 Typical Wiring Schemes Fig. 390
Page 287 and 288: 288 Typical Wiring Schemes Fig. 392
Page 289 and 290: 290 Fig. 394 Pump K-1001 / Autosamp
Page 291 and 292: 292 Index Device ID Autosampler 68,
Page 293 and 294: 294 Index R Rack configuration 243
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