ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

ChromGate® PDA Option 209
Spectrum Background Correction
A background correction can be performed if the current spectrum has
been extracted from an integrated peak. Select the right mouse
click/Operations/Background Correction command from the Spectrum
window. Then click on the spectrum on which you wish to perform the
calculation. A background correction for the displayed spectrum will be
performed based on the working chromatogram. The backgroundcorrected
spectrum is then displayed along with the original spectrum.
Please refer to the PDA Analysis and Calculations section for details
on how this calculation is performed.
Background Correction may only be performed once on a spectrum. If a
second background correction is attempted on an already corrected
spectrum, a message box is displayed and the operation ignored.
If the current spectrum has not been extracted from an integrated peak of
the working chromatogram, selecting this menu item will have no effect
on the spectrum.
Background subtraction must always be the first operation performed on
a spectrum. If a background correction is attempted on a spectrum after
another operation has already been performed (including operation
specified in the Spectrum tab of PDA Options), a message box is
displayed and the background correction request ignored.
Interpolate Spectrum
Spectrum Interpolation can be performed if the current spectrum has
been extracted from an integrated peak. Select the right mouse
click/Operations/Interpolate command from the Spectrum window.
Then click on the spectrum on which you wish to perform the calculation.
A 10:1 interpolation will be performed on the spectrum. The new
interpolated spectrum is then displayed along with the original spectrum.
Please refer to the PDA Analysis and Calculations section for details
on how this calculation is performed.
Refer to the Data Analysis and Calculations section for details on how
the values are computed.
Export
Selecting this menu item will display a File Save As dialog. When a valid
filename is entered and Ok is pressed, the currently displayed spectrum
will be exported as an ASCII data file. Refer to the Spectrum Export
section for details on the data file format.
Overlay Spectra
You can overlay spectra using the Actions/Overlay Spectra… command
from the Mixed View toolbar. When you select this option, a dialog
appears where you can enter the retention time from which you want to
extract a spectrum. You can also overlay spectra quickly by moving the
wavelength cursor in the Contour or Chromatogram view to the desired
wavelength. To remove the spectra you have added to the view, use the
Clear overlays command from the right mouse click menu in the
Spectrum view. You can also clear selected spectra using the Trace
Setup dialog accessed by doing a right mouse click in the view and then
selecting Properties.