ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

SEC Option 259
chromatography are distinctly different techniques, and cannot therefore
be performed simultaneously on the same instrument.)
In general, performing an SEC analysis involves the following steps.
1. Run SEC calibration standard(s) and use it to set up the calibration
information for the SEC samples (Method/SEC Setup).
2. Create a custom report to include the types of SEC reports and
plots you want for your analyses.
3. Create a sequence (if appropriate) and run your SEC samples.
Running an SEC Calibration Standard
Before you run your calibration standard(s) you must first set the
acquisition parameters so that all the data are correctly sampled and
saved on your disk. Do this using Instrument Setup. From the
instrument window for the chromatograph you intend to use for your SEC
run, click on the Instrument Setup button or select Method/Instrument
Setup from the menu.
Instrument Setup
The instrument setup portion of your method tells how you want to
acquire the data coming from your chromatograph.
The default acquisition data rate of 1 Hz is adequate for SEC runs.
You can adjust the "slice width" for slice reports independently in
the SEC setup part of the method
Detector 1…x
For each detector channel (x) configured on the instrument, define the
following acquisition information.
Acquisition Channel On
Select this box to turn the acquisition for this channel ON. If this box
is not selected, no data will be acquired for this channel.
Sampling
This is the rate at which data will be sampled by the system. You can
choose how you want to specify the sampling rate. When you select a
sampling rate, a prompt will appear indicating the narrowest peak
width for which this sampling rate will be adequate.
It is recommended that you use the Graphical Events Programming
to determine the optimum sampling rate for your chromatography.
Frequency
This selection is in Hz (samples per second). This is the selection for
most chromatography applications. Click on the down-arrow to get a
list of the frequencies available for the configuration of your system.
Period
When you select this type of sampling, you must select the number of
seconds (or milliseconds) between data points. Enter the value, then
select whether the period is in milliseconds (msec) or Seconds.
Run Time
Run Time determines the length of time data will be sampled