ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

206 ChromGate® PDA Option
Fig. 302
You can continue to select or change the peak. When you are finished,
click the Close button.
Purity View Properties
To set up the properties for the Purity View, do a right mouse click, and
select Properties.
By default, the Similarity View will use the current method settings. To
override the method settings, click the Override method settings box,
then enter the values you wish to use.
Wavelength range
Specify the wavelength range over which the purity calculations will be
performed, for example, from 200 nm to 400 nm.
Wavelength Step
Specify the wavelength spacing (in nm) to be used when purity
calculations are performed.
Purity threshold
The comparison of two spectra gives a Similarity Index (SI), the closer to
1.00 number is the more similar the spectra are. The purity threshold is
used to eliminate spectra that do not match. If the SI is greater than the
purity threshold, the spectra will be considered pure. In general, a
spectra with an SI greater than 0.9900 would have a high probability of
being the same as the apex spectra. A SI greater than 0.9000 but less
than 0.9900 shows some similarity but would need to be evaluated with
care and caution. SI less than 0.9000 should be considered non-similar.
Absorbance threshold
This value represents the percentage of peak height that spectra will
include in purity calculation. Spectra in sections of the peak that do not
exceed this threshold will not be included in the purity calculation. This
provides a method of eliminating spectra where the concentration of the
compound is so low that the solvent spectrum interferes.
Background compensation
Checking this box will cause spectra to be corrected for background
using the peak baseline prior to being used in the calculation of purity.
Similarity View Properties
To set up the properties for the Similarity View, do a right mouse click,
and select Properties.