ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

More documents

Recommendations

Info

Creating an Instrument Control Method 121 Fig. 172 Autosampler 3950 Stacked injections auxiliary trace Fig. 173 Autosampler 3950 Stacked injections trace For every stacked injection an entry for the sample preparation and the injection will be made. Fig. 174 Autosampler 3950 Stacked injections instrument activity log entry Instrument Setup – Triathlon/Endurance Autosampler The method parameters required for automatic operation of your autosampler are contained in the Method/Instrument Setup/Triathlon (Endurance) tab. The control parameters for the autosampler become part of your method and sequence files.
122 Creating an Instrument Control Method Fig. 175 Autosampler Triathlon setup window Standard Injection In the Standard Injection section you can enter parameters for the standard actions that can be performed with the autosampler. Injection Method Click one of the Flushed, Partial or µl Pick-up fields to select the injection method. The maximum programmable injection volumes are: Flushed loop: not programmable, is equal to the loop volume but needs more sample to fill the loop completely (3 x loop volume for loop volumes < 100 µl and 2 x loop volume for loop volumes > 100 µl) Partial loop fill: 50 % of the programmed loop volume µl Pick-up: Injection volume = (loop volume – 3 x needle volume) / 2 Syringe Speed and Scale Factor The aspirating speed of the syringe used in injection methods can be adapted depending on the viscosity of the samples (the choices are Low, Normal, and High). Alternatively, syringe speeds can be reduced by entering a scale factor (1 to 10). The syringe speed will be the scale factor multiplied by the syringe speed. The syringe speed during the washing or the rinsing procedures is not affected by this setting. Air Segment Check the Air Segment box to enable the use of an air segment between the sample and the wash solvent to reduce the amount of flush volume. Headspace Pressure Check the Headspace Pressure box to enable the use of headspace pressure in combination with the syringe to transport sample to the loop. The compressor will always be used during a wash procedure. The accuracy and reproducibility of the autosampler may decrease if headspace pressure is switched off. Needle Wash Check the Needle Wash box to enable a wash procedure. Flush Volume Enter the amount of sample in µl taken from a vial to preflush the needle before the loop is filled with the sample. The default value is 30 µl for an installed tube volume of 15 µl.
Page 1 and 2:
Software ChromGate ® Chromatograph
Page 3 and 4:
4 CONTENTS Configuration - Autosamp
Page 5 and 6:
6 CONTENTS Peak Table .............
Page 7 and 8:
8 Installation Guide ChromGate® 3.
Page 9 and 10:
10 Installation Guide ChromGate® 3
Page 11 and 12:
12 Installation Guide ChromGate® 3
Page 13 and 14:
14 Short guide ChromGate®, KNAUER
Page 15 and 16:
Page 17 and 18:
Page 19 and 20:
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
32 Setup and Control of Knauer HPLC
Page 33 and 34:
Page 35 and 36:
Page 37 and 38:
Page 39 and 40:
Page 41 and 42:
Page 43 and 44:
Page 45 and 46:
Page 47 and 48:
Page 49 and 50:
Page 51 and 52:
Page 53 and 54:
Page 55 and 56:
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
Page 63 and 64:
Page 65 and 66:
Page 67 and 68:
Page 69 and 70: 70 Setup and Control of Knauer HPLC
Page 83 and 84: 84 Creating an Instrument Control M
Page 99 and 100: 100 Creating an Instrument Control
Page 119: 120 Creating an Instrument Control
Page 169 and 170: 170 Knauer Instrument Control Metho
Page 171 and 172:
172 Knauer Instrument Control Metho
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
Page 183 and 184:
Page 185 and 186:
186 ChromGate® System Suitability
Page 187 and 188:
188 ChromGate® System Suitability
Page 189 and 190:
190 ChromGate® PDA Option Fig. 283
Page 191 and 192:
192 ChromGate® PDA Option calculat
Page 193 and 194:
194 ChromGate® PDA Option (to the
Page 195 and 196:
196 ChromGate® PDA Option PDA View
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Page 205 and 206:
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
216 ChromGate® PDA Option Note tha
Page 217 and 218:
Page 219 and 220:
Page 221 and 222:
222 PDA Analysis and Calculations c
Page 223 and 224:
224 PDA Analysis and Calculations s
Page 225 and 226:
226 PDA Analysis and Calculations u
Page 227 and 228:
228 PDA Analysis and Calculations 3
Page 229 and 230:
230 ChromGate® Preparative Option
Page 231 and 232:
Page 233 and 234:
Page 235 and 236:
Page 237 and 238:
Page 239 and 240:
Page 241 and 242:
Page 243 and 244:
Page 245 and 246:
Page 247 and 248:
Page 249 and 250:
Page 251 and 252:
Page 253 and 254:
Page 255 and 256:
Page 257 and 258:
258 SEC Option Universal Calibratio
Page 259 and 260:
260 SEC Option Fig. 369 Acquisition
Page 261 and 262:
262 SEC Option Amount Values In thi
Page 263 and 264:
264 SEC Option Fig. 373 SEC peak an
Page 265 and 266:
266 SEC Option Fig. 376 Fig. 377 Yo
Page 267 and 268:
268 SEC Option Fig. 379 Enable Anal
Page 269 and 270:
270 SEC Option Export settings Clic
Page 271 and 272:
272 SEC Option All samples analyzed
Page 273 and 274:
274 SEC Option Horizontal Baseline
Page 275 and 276:
276 SEC Option Intrinsic Viscosity
Page 277 and 278:
278 SEC Option Fig. 383 information
Page 279 and 280:
280 SEC Option Fig. 386 Fig. 387 Se
Page 281 and 282:
282 SEC Option single variable sear
Page 283 and 284:
284 Typical Wiring Schemes Typical
Page 285 and 286:
286 Typical Wiring Schemes Fig. 390
Page 287 and 288:
288 Typical Wiring Schemes Fig. 392
Page 289 and 290:
290 Fig. 394 Pump K-1001 / Autosamp
Page 291 and 292:
292 Index Device ID Autosampler 68,
Page 293 and 294:
294 Index R Rack configuration 243
Page 295:
© ® Wissenschaftliche Gerätebau
show all

ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

Create successful ePaper yourself

Delete template?

Save as template?