ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

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ChromGate® PDA Option 193 2. For each spectrum in the peak, a corresponding background spectrum is generated by linear interpolation between the baseline start and baseline stop spectra. 3. This background spectrum is subtracted from the original spectrum. The baseline start and stop times that are used in calculating background compensation are based on the detected peaks for the channel currently selected. Interpolate spectra Select this check box to automatically perform 10:1 interpolation of spectra displayed in the spectrum window using a cubic spline curve fit. This interpolation is performed after the applying any spectral filtering option (1st derivative, 2nd derivative or smooth) to the display spectrum. Interpolated spectra may not be stored or added to a library. The peak spectrum is defined as This specifies if and how spectra extracted from the 3D data are averaged before being exported or used in the Spectrum Report. Spectrum Averaging is the calculation of a spectrum whose absorbance values are the average of the absorbencies. The peak apex spectrum When this is selected, the spectrum at the apex of the peak will be used, without averaging. The avg. of upslope, apex and down slope spectra When this is selected, three spectra (the spectra at the upslope inflection point, the apex and the down slope inflection point) are averaged. The avg. of every n spectra across the peak When this is selected, enter a value for n. Every nth spectrum from the peak is extracted, beginning at the start of the peak and continuing to the end of the peak. These extracted spectra are averaged and become the spectrum that is reported or exported. Per-peak spectrum calculations Checking any of these boxes indicates that the indicated value will be calculated on a per-peak basis during analysis. The result of this calculation will then be available in reports and as chromatogram annotations. Unchecking values that are not of interest will speed up analysis. If a box is unchecked and the field appears in a run report, it will be reported as zero Similarity Checking this box causes the Peak Apex Similarity to the reference spectrum to be calculated on a per-peak basis during analysis. The result of this calculation will then be available in reports and as chromatogram annotations. If this box is unchecked and a report contains the field, a zero will be reported. Peak Apex Similarity applies only to named peaks and its use requires that a reference spectrum be included in the peak table. Upslope Similarity Checking this box causes upslope similarity to be calculated on a perpeak basis during analysis. Upslope similarity compares the peak apex spectrum to the spectrum at the peak inflection point on the upslope side
194 ChromGate® PDA Option (to the left of the apex). The result of this calculation will then be available in reports and as chromatogram annotations. If this box is unchecked and a report contains the field, a zero will be reported. Down slope Similarity Checking this box causes down slope similarity to be calculated on a perpeak basis during analysis. Down slope similarity compares the peak apex spectrum to the spectrum at the peak inflection point on the down slope side (to the right of the apex). The result of this calculation will then be available in reports and as chromatogram annotations. If this box is unchecked and a report contains the field, a zero will be reported. Lambda max Checking this box indicates causes the lambda max (wavelength at which the highest absorbance occurs) to be computed for each peak of each multi-chromatogram extracted from the 3D data during analysis. The result of this calculation will then be available in reports and as chromatogram annotations. If this box is unchecked and a report contains the field, a zero will be reported. Lambda max Calculation range This allows the user to restrict the wavelength range over which the Spectrum Max Plot will be calculated. Default is the detector acquisition range. PDA Options Multi-Chromatogram Fig. 286 The Multi-Chromatogram tab is used to specify data channels within the 3D data for integration and quantization. It allows you to select wavelengths to display on the Multi-Chromatogram plot. When you select an Enabled box, enter a wavelength and bandwidth to be used for one plot. After you have set up several wavelengths, you can disable them temporarily by clicking the Enabled box such that the check mark is not displayed. The Multi-Chromatogram display is selected from the View Gallery. The Multi-Chromatogram view is not functional unless one or more valid wavelengths are enabled in the Multi-Chromatogram Definition tab. Enabled To enable a wavelength, click the checkbox. To disable a wavelength, click the checkbox again to remove the checkmark. Only enabled wavelengths will appear in the multi-chromatogram view.
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Software ChromGate ® Chromatograph
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4 CONTENTS Configuration - Autosamp
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6 CONTENTS Peak Table .............
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8 Installation Guide ChromGate® 3.
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10 Installation Guide ChromGate® 3
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12 Installation Guide ChromGate® 3
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14 Short guide ChromGate®, KNAUER
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32 Setup and Control of Knauer HPLC
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84 Creating an Instrument Control M
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100 Creating an Instrument Control
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Page 141 and 142: 142 Creating an Instrument Control
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244 ChromGate® Preparative Option
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258 SEC Option Universal Calibratio
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260 SEC Option Fig. 369 Acquisition
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262 SEC Option Amount Values In thi
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264 SEC Option Fig. 373 SEC peak an
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266 SEC Option Fig. 376 Fig. 377 Yo
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268 SEC Option Fig. 379 Enable Anal
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270 SEC Option Export settings Clic
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272 SEC Option All samples analyzed
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274 SEC Option Horizontal Baseline
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276 SEC Option Intrinsic Viscosity
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278 SEC Option Fig. 383 information
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280 SEC Option Fig. 386 Fig. 387 Se
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282 SEC Option single variable sear
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284 Typical Wiring Schemes Typical
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286 Typical Wiring Schemes Fig. 390
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288 Typical Wiring Schemes Fig. 392
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290 Fig. 394 Pump K-1001 / Autosamp
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292 Index Device ID Autosampler 68,
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294 Index R Rack configuration 243
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ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

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