ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

SEC Option 269
Horizontal Baseline
Select this button if you wish the SEC baseline to be drawn as a
horizontal line forward from the Baseline Start Time to the Stop Time.
The horizontal baseline stops when it crosses the data trace. A threshold
value in microvolt must be specified to ignore any detector baseline noise
in the trace that would cause the horizontal projection to stop when it
encounters the noise in the trace. If the Horizontal Baseline reaches the
end of the detected peak before the programmed Stop Time, the range
will stop where the Horizontal Baseline crosses the data trace.
Sample Data
If you have selected Narrow standards calibration, this field will appear
empty.
Reference Peak Time (min)
Select this box if you are using a retention time reference peak to adjust
for changes in flow rate. All elution times/volumes used in calculations
will be offset by the time difference between the value entered here and
the time of the reference peak in the sample. Enter the expected
retention time for the reference peak, in minutes.
Reference Window (%)
If you are using a reference peak, enter a value for the % window for the
reference peak. A peak falling within +/- this % of the expected retention
time for the reference peak will be considered the reference peak. (If two
peaks fall within the window, the peak closest to the center will be chosen
as the retention time reference.)
Fit Type
Select a calibration curve fit type from the drop-down list. The list
includes: Point-to-point, Linear, Quadratic, Cubic, 4th Degree
Polynomial, and Spline.
Goodness of Fit
The calibration curve for the data in the SEC Setup will be displayed in
the calibration curve window. The r 2 value, which represents goodness
of fit of the calibration points to the curve selected, will be displayed
above the curve. A value of 1.00 indicates perfect fit. Select the Time or
Volume button at the bottom of the calibration curve to display the x-axis
of the curve as either retention time in minutes or retention volume. If
you select volume, enter the flow rate in ml/min.
Remove calibration curve outliers
To temporarily remove an outlier from the calibration curve, click on the
point with your mouse. It will change color and the curve will be recalculated
with the point removed.
Time/Volume
Select the mode of viewing for your calibration curve: Time or Volume. If
you select Volume, you must enter the flow rate so that the curve is
displayed correctly.
Print
Click this button to print your current SEC method information.