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ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

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96 Creating an Instrument Control Method<br />

Used Channels:<br />

Check the box(es) to turn the acquisition for the selected channel(s) on. If<br />

the box is not selected, no data will be acquired and no time program will<br />

be executed for this channel. As many channels are activated (at least<br />

one) as many wavelength columns appear in the spreadsheet below. You<br />

can only enable the number of channels you have activated in the<br />

instrument configuration setup of the device.<br />

The wavelength table is a spreadsheet to enter your time (detector)<br />

program. In the first row (at time 0.00 min), enter the initial wavelength(s)<br />

in nm. For up to 50 steps (time in minutes), you can then define the<br />

detector parameters. The editing principle including programming the<br />

digital outs is the same as for the time (gradient) program, described in<br />

the chapter Instrument Setup – Pumps, Pump Program.<br />

Autozero at start<br />

Check the Autozero field, if you wish the signal to be zeroed at<br />

measurement start (to define the current signal as the baseline). You<br />

cannot disable this option if you are acquiring 3D data in the advanced<br />

mode of the detector.<br />

Autozero at WL change<br />

Check this option if you wish the signal to be zeroed at the moment the<br />

wavelength is changed (to define the current signal as the baseline).<br />

Lamp OFF at the end of run<br />

Check the Lamp OFF box to switch off the detector lamp after a<br />

measurement is finished. This option may be used for the shutdown<br />

methods.<br />

You should switch the lamp on either manually or from the direct<br />

control window before you start the next working session. After<br />

power up, allow 30 minutes for the lamp to stabilize before running<br />

samples that may require sensitive measurements.<br />

Scan, nm [Range 190 – 740 nm]<br />

This section allows you to specify the scan spectral interval, in nm.<br />

Start<br />

Enter the start wavelength for the scan in nm.<br />

End<br />

Enter the end wavelength for the scan in nm.<br />

The scan section limits Start and End are important even if no PDA<br />

option is available. All measuring wavelengths in the used channels<br />

1 to 4 must be within these limits.<br />

Acquire 3D data<br />

You have access to this selection field only with the PDA option. The<br />

<strong>Advanced</strong>… button becomes accessible if the Acquire 3D data check<br />

mark is set.<br />

Fig. 140 Detector K-2600 setup window with PDA option<br />

A click on the <strong>Advanced</strong>… button will open the following dialog box.

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