ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

22 Short guide ChromGate®, KNAUER Instrument Control
Fig. 16 Peak table
- Enter the Reference ID#, which is the Peak ID#s from the peak
table. This number is obtained from the Peak Table. Leave it
blank until you have generated the peak table, then enter the
value directly into the Peaks / Groups table.
- Indicate the desired basis for the calculation of the retention
time window and the desired value.
7. Repeat the above step for each compound in the standard. When
you have entered information for all standards, press Done.
8. Press the Peaks / Groups Table button or select the menu
sequence Method – Peaks / Groups … to present the calibration
peak table. Each peak that you have defined will appear as a row
in the table, along with the retention time and other parameters that
you have entered.
9. Enter all values needed for the calibration as the concentration for
all levels of your compounds.
10. If the table is complete, close Peak Table dialog box by clicking on
the x in the upper right corner.
11. Save the method.
12. Select Analysis/Analysis Single Level Calibration from the
menu bar to present the following dialog box.
Fig. 17 Analysis Single Level Calibration Window
13. Enter the sample identification used for the standard.
14. Enter the method that you want to calibrate and the data path
name for the data file.
15. Enter the name of the calibration data file.
16. Click on the Calibration check box and enter a 1 for Calibration
level. If the method is presently not calibrated, it is not necessary to
check any of the boxes (but if you want to be certain, select the
Clear all calibration check box).
17. Repeat the steps 12. to 16. for all levels you want to calibrate.
Enter the appropriate calibration level into the Calibration level field