ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

ChromGate® Preparative Option 237
Adjust FRC table after stacked injection
This option allows you to copy the already executed part of the fraction
table for the next injection, if a stacked injection is performed. In this case
you will have the same collection conditions for every injection without
any manual intervention. This makes sense, if you use Single Event
parameters, which otherwise should have to be programmed separately
for every injection. If you use the peak recognition mode, just program the
collection condition for the whole run time, the Adjust FRC table option
leave unchecked.
Pump
If more than one pump is configured in the system, select the used one
from the drop down list for volume and delay calculations.
Update Flow
The set flow of the selected pump after the last actualization will be
displayed. If the flow is not constant for the whole method, the value of
0.000 will be shown. Please enter then the flow rate for the time the
fractions will be collected. The flow is important to calculate the time
delay between detecting a component in the detector and the approach
to the fraction valve as well as the filling level of the vials.
Fraction Collection Program
The editing principles are again the same as it was described for the
pump gradient programming. Some fields are free for entering data
whereas others are either only informative without access or you can
select the possible options via a drop down list.
Exp. Time (min)
Enter the desired time values.
Mode
Fig. 330 Fractionation mode selection
The single event option is to select for programming a detailed exact
fractionation program, whereas the peak recognition is preferably for
use in method development. Every new detected peak according to
the defined thresholds (see Parameters on page 238) will be collected
in the next empty vial. The selected mode will influence the further
setting possibilities.
Event
Fig. 331 Event definition
The possible options depend on the selected mode: