ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

SEC Option 271
Std Mark-Houwink k
If you have selected Mark-Houwink for the Use Constants field, enter the
k value for the standard in this field.
Std Mark-Houwink a
If you have selected Mark-Houwink for the Use Constants field, enter the
a value for the standard in this field.
Intrinsic Viscosity
If you have selected Intrinsic Viscosity for the Use Constants field, enter
the value here. Otherwise this field is not active.
Time Slice
Select a time slice width (in seconds) for the calculations. The slice width
will determine the frequency of Molecular Mass fractions reported. If a
Slice Report is generated, it will be based on the slice width you select
here. The software will force this number to be an integral of the data
collection rate during the analysis.
Baseline Start and Stop Times
The baseline start and stop times (in minutes) can be entered manually
or selected graphically using the SEC buttons on the chromatogram
toolbar.
Results Range Start and Stop
One or more ranges of the chromatogram must be defined to be used in
calculation and reporting of the Molecular Mass distribution. Each range
is determined by the results range start and stop values. The times (in
minutes) can be entered manually or graphically using a stored
chromatogram.
Horizontal Baseline
Select this button if you wish the SEC baseline to be drawn as a
horizontal line forward from the Baseline Start Time to the Stop Time.
The horizontal baseline stops when it crosses the data trace. A threshold
value in microvolt must be specified to ignore any detector baseline noise
in the trace that would cause the horizontal projection to stop when it
encounters the noise in the trace. If the Horizontal Baseline reaches the
end of the detected peak before the programmed Stop Time, the range
will stop where the Horizontal Baseline crosses the data trace.
Calibration
Select Universal for the calibration type.
If Universal Calibration is selected, the Mark-Houwink constants or
intrinsic viscosity of the sample(s) must be entered to display an
adjusted calibration curve.
Sample Data
Use this area to input K and a or measured intrinsic viscosity values for
your samples. These values in conjunction with the Mark-Houwink
constants/intrinsic viscosity specified in the calibration table are used to
adjust the calibration curve to better represent the sample being
analyzed.