ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

PDA Analysis and Calculations 223
� Each multi-chromatogram channel is an average of the
absorbencies monitored at each wavelength in the wavelength
range. The wavelength range is equal to the selected wavelength +/-
one half of the bandwidth.
Working Chromatogram
The following apply to the working chromatogram:
� In the Channel Selection drop-down list, a single entry is added that
identifies the channel as applying to the working chromatogram.
� This channel has a separate Integration Events Table, Manual
Integration Table, Export Table and Performance Table associated
with it. These are separate from the tables of the Multi-
Chromatogram Channels (above).
� When an analysis is performed, the working chromatogram will
automatically be analyzed.
� The working chromatogram is an average of the absorbencies
monitored at each wavelength in the wavelength range. The
wavelength range is equal to the selected wavelength +/- one half of
the bandwidth.
� The working chromatogram is not available as a trace in Custom
Reports.
Spectra Extracted from the 3D Data
Two types of spectra may be extracted from the 3D data:
Analysis Spectra
� Analysis Spectra: One or more spectra automatically extracted from
the 3D data during analysis and used for peak identification,
similarity and/or library search.
� Working Spectrum: A spectrum extracted from the Contour plot on
the Mixed View display. (This chromatogram is displayed in the
Spectrum View)
� Specific Analysis-related capabilities are for these spectra are
detailed below.
The following applies to the analysis spectra:
� Prior to being used elsewhere, all analysis spectra extracted from
the 3D data are filtered according to the settings on the Spectrum
tab of PDA Options.
Working Spectrum
The following apply to the working spectrum:
� Working spectra extracted from the 3D data are never filtered
according to the settings on the Spectrum tab of PDA Options.
� The working Spectrum is not available as a trace in Custom Reports.
Background Correction
Background Correction may only be performed on a spectrum as a result
of the settings on the Spectrum tab of PDA Options, the settings on the
Purity tab of PDA Options, or by setting the properties of the Peak Purity
Pane.
Background subtraction is always the first operation performed on a
spectrum. If a background correction is attempted on a spectrum after
another operation has already been performed (including an operation