ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

SEC Option 275
SEC Setup Broad Range 2 Calibration
Fig. 382
If you select Broad Range 2 for the Calibration Type, the following
screen will appear.
Enable Analysis
Click this box to enable SEC calculations for the current channel. Each
channel can have its own analysis conditions.
Molecular Mass
Enter a series of Molecular Mass s that will be used in determining the
broad range calibration curve.
Average Time (min)
Enter the time in minutes for the Molecular Mass specified. If you have
used the graphical Define SEC Peaks command, the peak times will be
entered already for you. Note: the time is converted to seconds for
calibration and Molecular Mass calculations.
Use Constants
Select the constants to be used in the calibration - either Mark-Houwink
or Viscosity from the drop-down selection. Based on your selection, the
appropriate columns in your spreadsheet will become active.
Std Mark-Houwink k
If you have selected Mark-Houwink for the Use Constants field, enter the
k value for the standard in this field.
Std Mark-Houwink a
If you have selected Mark-Houwink for the Use Constants field, enter the
a value for the standard in this field.