ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...
ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...
ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...
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232 ChromGate® Preparative Option<br />
These settings enable someone to consider the time delay for<br />
transporting the sample from the flow cell to the collector valve. If the<br />
option Use recycling valve is checked, the capillary from the flow cell via<br />
the recycling valve to the fractionation valve must be taken into account.<br />
If you activate the option Fixed time you can enter this time delay<br />
directly. This value will be valid, even if the flow rate is changed!<br />
Entering the Capillary Volume directly or calculating it by entering the<br />
Capillary Length and Inner Diameter, the delay time will be adapted to<br />
the programmed flow rate of the method.<br />
Solvent and/or Peak Recycling<br />
For an optional solvent and/or peak recycling, for most fraction collectors<br />
an additional recycling valve is necessary. As a recycling valve only a<br />
Knauer 6-port valve can be used. The Knauer fraction collector 3050<br />
includes a valve for recycling. For a multi valve fraction collector some<br />
ports of the fractionation valve can be defined for the recycling.<br />
To define a separate recycling valve, check the option Use recycling<br />
valve and click on the button. For the separate recycling valve a COM<br />
port or the IP address is to define. The settings for the volumetric delay<br />
will be displayed without access for changing. The settings are valid as<br />
well for the recycling valve as for the fractionation valve. Therefore, the<br />
capillary between the recycling valve and the fraction collector should be<br />
as short as possible.<br />
Only for the Knauer fraction collector 3050 and the multi valve fraction<br />
collectors the option Use own ports for recycling will be displayed. If<br />
you want to use this option, click into the check box for this option. For<br />
the multi valve fraction collector you will find, that the number of available<br />
ports for fractionation will be reduced by 2. Additionally the ports numbers<br />
for solvent and peak recycling will be shown. It is not possible only to<br />
enable one of the recycling options.<br />
Fig. 322 Recycling valve setup
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