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ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

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ChromGate® Preparative Option 253<br />

Number of Injections<br />

Enter the number of stacked injections. If you, e.g., enter “5”, two stacked<br />

injections will be made; in addition of the initial injection 6 injections at all<br />

will be made per run. Please make sure that you have enough samples<br />

for the entered number of injection.<br />

Injection Volume<br />

Enter the desired injection volume in ml.<br />

Injection Flow<br />

Enter the flow rate of the injection pump during the injection. If no<br />

injection is prepared, the pump is stopped. During a run the injection<br />

module’s pump can only be controlled using the option “Direct control<br />

during a run” (please refer the manual’s appropriate chapter Runtime<br />

Settings, page 141).<br />

Injection Duration<br />

The duration of the injection is calculated from the injection volume and<br />

the injection flow. The reduced flow during start and stop of the pump is<br />

taken into account with 6 seconds in sum.<br />

Time between Injections (min)<br />

Enter the time between the injections. The value must be higher than the<br />

time calculated for the injection duration.<br />

Click the Apply-button to apply the settings into the Stacked injections<br />

table.<br />

Fig. 363 Injection module Stacked injection table<br />

If you make a right mouse click on the table, you can select<br />

- Fill Down, to fill the table with the same settings as in the selected line<br />

- Insert Line, to add a new line<br />

- Delete Line, to delete the selected line<br />

- Delete Lines, to delete all selected lines<br />

- Fill Table…, will open the Stacked injection setup again. The new table<br />

will overwrite the existing one. If you select the command Fill table to<br />

open the setup dialog for the stacked injection table, the window will<br />

show default values for all parameters, beside the sample preparation<br />

time.<br />

Click on OK to close the Stacked Injections table window; the injection<br />

module instrument setup window will be shown.<br />

In the Aux Traces tab a trace “Stacked Inj. [Inj. Module]” can be enabled.<br />

The trace will show the start of the sample preparation and the stacked<br />

injection.

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