ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

126 Creating an Instrument Control Method
starts. The programmed volume of wash solvent is used to wash the
needle at the wash position.
The wash position may be contaminated with the contents of the
buffer tubing, which may generate cross-contamination. To
prevent contamination of the wash position, program a
“dispense to waste” action before programming a wash action.
Valve
The positions of the installed high pressure valves are programmed in
this screen. The injector valve has two positions: INJECT and LOAD.
If the ISS option is installed, the two valves have the two positions: 1-6
and 1-2.
Wait
The WAIT action can be used to let the autosampler wait for a
programmed time with maximum of 9 h 59 min 59 sec.
During WAIT the needle turns back to home position if the next
action is not at the same position. Should the needle be required
to remain in the current position, it is necessary to program a
dummy step after the WAIT in which an aspirate or dispense
action of 0 µl is programmed at the actual position.
Compressor
With the compressor action the compressor is activated to transfer air
pressure to a vial. The compressor will stay active until it is switched
OFF. The compressor will be automatically switched off at the end of
the needle wash routine if a needle wash is used.
Auxiliaries
With the Auxiliaries action the four standard auxiliaries (contact
closures) can be controlled (see time base events).
Wait for Input
The autosampler will wait for one of the four inputs to become HIGH
or LOW before continuing with the next step.
Prog. Output
The Prog. Output action is used to define two programmable outputs
(contact closures). These are similar to the auxiliaries.
Code Output
The Code Output action is used to program the output to the
connector P3 TIMED OUTPUTS on the rear panel of the autosampler.
This is a HEX output in the range 0 to 15 (F).
Markers
The markers normally generated by the autosampler are not active in
the user defined method, but can be programmed in this screen. The
choices are: Inject, Vial, and Labeled.
Markers must be programmed, because they are not automatically
generated as in the regular injection method. Refer to the
Triathlon/Endurance User Manual for the cable connections.
SSV Port
Program the solvent selection valve (SSV) port position, range 1 to 6.
Repeat
The last x steps will be repeated y times.
Button functions:
Click the Add button to complete the operation.