n - Кафедра Прикладная биотехнология

plotted of % phenol against the square of the mean diameter of the clear zone. The antibacterial activity
of honeys was evaluated by measuring the zone around the well. To allow for the dilution and density
of honey this figure could multiplied by a factor of 4.69 and the activity was then expressed as the
equivalent phenol concentration (% w/v). The factor of 4.69 is based on a mean honey density of
1.35g/ml. The data from zones of inhibition around each phenol standard after adding of increasing
percentage phenol used for preapearing standard graph.
Percentage antioxidant activity of honeys was calculated using the equation by
method of Al-Mamary et al. (Al-Mamary et al., 2002). Before analyses of antioxidant
activity each sample was diluted with distilled water in the ratio 1:10 (w/v) and antioxidant
activities from sample solutions (100 �l) were assayed in vitro by calculation of
inhibition in liver homogenate from pig with adding oxidative solution (50 mol/l FeSO4;
1 mmol/l KH2PO4; 0,2 mmol/l ascorbic acid in 0,15 M Tris-HCL buffer with рН 7,4),
and reading extinction’s at 532 nm with spectrophotometer (SP 870).
The antibacterial activity (expressed in mm of diameter of zone for inhibition), were
compared with antioxidant activity (%), (table). Statistical analyses were performed by
t-тест (Statmost TM for Windows).
Results from our first experiment shows that in all cases before adding of catalase
solution we found total inhibition (partial antibacterial activity), to referent strain of
Staphylococcus aureus (ATCC 9144), (table). The lowest value (29,45 � 1,01 mm) was
found from acacia honeys and the highest value from investigated oak honeydew (36,90
� 1,74 mm) honeys. Our results were similar to these of Portuguese scientists which
point that all natural honeys possessed peroxide antibacterial activity (activity destroyed
by catalase), (Henriques et al., 2005).
Our results from antioxidant activity were with compliance with data from other scientists
(Kucuk et al., 2007), which point that the antioxidant activity was found to be
related to the sample concentrations. In tested types of honeys between antibacterial and
antioxidant activities (p