Liečivé rastliny v meniacich sa environmentálnych podmienkach

Vliv biotických a abiotických stresorů na vlastnosti rostlin 11.5.2005
Summary
Influence of heavy metals (Cd-EDTA, Pb-EDTA) on early somatic embryos of spruce (Picea
spp.)
Key words: heavy metals, cadmium, lead, EDTA, somatic embryo, picea
A growth, viability and morphology of early somatic embryos of spruce (ESES) are influenced by a presence of
heavy metals ions in a cultivation medium. That is why we studied the heavy metals influence on clones of 2/32 and
3/5H of Norway spruce and clone of Blue spruce. We added Pb-EDTA and Cd-EDTA at concentrations of 50 μM,
250 μM and 500 μM to LP/2 cultivation medium. Consequently we observed the growth of the studied clones by
weighing and image analysis. For the registration of images, a charge-coupled device (CCD) camera in connection
with a personal computer and software of GRAB-IT and Image-Pro was used. The difference between increase in
embryos area and its weight rose with increasing concentration of heavy metals. The weight of ESES was higher at
higher concentrations of heavy metals in comparison with control embryos, which had same clusters area. We found
out that increase in ESES clusters area decreased with increasing lead concentration, except the highest
concentration, where the increase was highest. We observed the similar behaviour at the all of the studied clones.
The viability of the embryos was estimated both by double staining (fluoresceine diacetate and propidium iodide) and
by activity of intracellular esterases. The number of dead cells of early somatic embryos increased with increasing
lead concentration.
Poděkování
Příspěvek vznikl za podpory IGA MZLU 250061/2005, GAČR 525/04/P132 a FRVŠ 2348/F4a.
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