LieÃ„ÂivÃƒÂ© rastliny v meniacich sa environmentÃƒÂ¡lnych podmienkach

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Vliv biotických a abiotických stresorů na vlastnosti rostlin 11.5.2005 500 μM byl ob<strong>sa</strong>h GSSG výrazně vyšší než u nižších aplikovaných dávek kadmia. Tento výrazný nárůst ob<strong>sa</strong>hu GSSG pravděpodobně souvisí se vznikem kyslíkových radikálů. Summary High performance liquid chromatography with electrochemical detection as a tool for studying of plants thiol compounds Key words: glutathione – phytochelatins – cadmium – high performance liquid chromatography – electrochemical detection We used the high performance liquid chromatography with electrochemical detection for the determination of thiol compounds in maize plants treated by different concentrations of cadmium (0, 50, 100, 150, 200, 400 and 500 μM) for six days. Influence of cadmium on maize plants was characterized by marked growth depression and by changes of leaves (necrosis). It clearly follows from the obtained HPLC-ED results that amounts of glutathione (reduced and oxidized) and phytochelatins increase with increasing applied cadmium concentration and cultivation time. In addition the GSH : GSSG ratio change with increasing time of cultivation. Poděkování Práce byla podporována granty: GA ČR č. 524/04/P132, IGA MZLU 250061/2005 a FRVŠ 2348/F4a. Literatura /1/ CAZALE, A.-C., CLEMENS, S.: FEBS Lett., 507, 215–219 (2001). /2/ COBBETT, C. S.: Trends Plant Sci., 4, 335–337 (1999). /3/ COBBETT, C. S.: Plant Physiol., 123, 825–832 (2000). /4/ COBBETT, C. S.: Curr. Opin. Plant Biol., 3, 211–216 (2000). /5/ DABRIO, M., RODRÍGUEZ, A. R., BORDIN, G., BEBIANNO, M. J., DE LEY, M., ŠESTÁKOVÁ, I., VAšÁK, M., NORDBERG, M.: J. Inorg. Biochem., 88, 123–134 (2002). /6/ ŠESTÁKOVÁ, I., VODIČKOVÁ, H., MADER, P.: Electroanalysis, 10, 764–770 (1998). /7/ KLEJDUS, B., KIZEK, R., VACEK, J., ZEHNÁLEK, J., TRNKOVÁ, L., KUBÁŇ, V.: J. Chromatogr. B, 806, 101-111 (2004). /8/ ZENK, M. H.: Gene, 179, 21-30. (1996). /9/ DI TOPPI, L. S., GABBRIELLI, R.: Environ. Exp. Botany, 41, 105-130 (1999). /10/ VACEK, J., PETREK, J., KIZEK, R., HAVEL, L., KLEJDUS, B., TRNKOVA, L., JELEN, F.: Bioelectrochemistry, 63, 347-351 (2004). /11/ VACEK, J., KIZEK, R., KLEJDUS, B., HAVEL, L.: Biol. Listy, 68, 133-153 (2003). /12/ VACEK, J., PETŘEK, J., HAVEL, L., KOUTNÁ, D., ADAM, V., TRNKOVÁ, L., KIZEK, R.: In: VI. Pracovní setkání biochemiků a molekulárních biologů, Brno, (2002). /13/ KLEJDUS, B., ZEHNALEK, J., ADAM, V., PETREK, J., KIZEK, R., VACEK, J., TRNKOVA, L., ROZIK, R., HAVEL, L., KUBAN, V.: Anal. Chim. Acta, 520, 57-67 (2004). /14/ ZEHNALEK, J., ADAM, V., KIZEK, R.: Listy Cukrov, 120, 222-224 (2004). /15/ ZEHNALEK, J., VACEK, J., KIZEK, R.: Listy Cukrov, 120, 220-221 (2004). /16/ GRILL, E., WINNACKER, E.-L., ZENK, M. H.: Proc. Natl. Acad. Sci. USA, 84, 439–443 (1987). 295
Vliv biotických a abiotických stresorů na vlastnosti rostlin 11.5.2005 /17/ GRILL, E., LOFFLER, S., WINNACKER, E.-L., ZENK, M. H.: Proc. Natl. Acad. Sci. USA, 86, 6838–6842 (1989). /18/ GRILL, E., WINNACKER, E.-L., ZENK, M. H.: Methods Enzymol., 205, 333–341 (1991). /19/ GRILL, D., TAUSZ, M., KOK, L. J. D., Plant Ecophysiology, Kluwer Academic Publishers, Dordrecht, Boston, London, 2001. /20/ KLOUDA, P., Moderní analytické chemie, Pavel Klouda, Ostrava, 2003. /21/ GARAJ, J., BERČÍK, J., BUSTIN, D., ČERŇÁK, J., ŠTEFANEC, J., TRAITER, M., Fyzikálne a fyzikálnochemické analytické metódy, Vydavatelstvo technickej a ekenomickej literatúry, Bratislava, 1977. /22/ SOMMER, L., ŠIMEK, Z., VOZNICA, P., Analytická chemie II, Vutium, Brno, 2000. /23/ INOUHE, M., ITO, R., ITO, S., SASADA, N., TOHOYAMA, H., JOHO, M.: Plant Physiol., 123, 1029-1036 (2000). /24/ INOUHE, M., NINOMIYA, S., TOHOYAMA, H., JOHO, M., MURAYAMA, T.: J. Plant. Res., 107, 201-207 (1994). 296
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LieÃ„ÂivÃƒÂ© rastliny v meniacich sa environmentÃƒÂ¡lnych podmienkach

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