Liečivé rastliny v meniacich sa environmentálnych podmienkach

Vliv abiotických a biotických stresorů na vlastnosti rostlin 11.5.2005
Cell cell death induced by simultaneous action of nitric oxide
and hydrogen peroxide
Key words: potassium ferricyanide - sodium nitroprusside - nitric oxide - hydrogen peroxide -
programmed cell death - tobacco BY-2
Summary
In plants interplay between synthesis of nitric oxide (NO) and reactive oxygen species (ROS) is
decisive for the induction of cell death in some cases. This effect was also demonstrated using donors of NO
and ROS. The aim of our work was to verify the specific effect of sodium nitroprusside (SNP; NO donor) on
the cell viability, morphology and biochemical characteristics, if cells are subjected to SNP and H 2 O 2
simultaneously. Cell suspensions of tobacco were treated by SNP, potassium ferricyanide (PFC, analogue of
SNP which can not release NO) and glucose with glucose oxidase (GGO, H 2 O 2 donor), respectively, and
moreover by combination of GGO with SNP or PFC. Entirely combination of SNP and GGO induced cell
death. SNP possessed NO specific effect in this case, although the ferricyanide itself influenced the
experimental system. During the cell decay chromatin condensation took a place, but DNA was only slightly
degraded. Organelles with low internal pH were detected within cells. In addition the number of vacuoles
decreased and small vesicles appeared in the cytoplasm.
Poděkování
Příspěvek vznikl za podpory IGA MZLU 250061/2005, GAČR 525/04/P132 a FRVŠ 2348/F4a.
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