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Liečivé rastliny v meniacich sa environmentálnych podmienkach

Liečivé rastliny v meniacich sa environmentálnych podmienkach

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Vliv biotických a abiotických stresorů na vlastnosti rostlin<br />

11.5.2005<br />

/8/ Tarkowská, D., Doležal, K., Tarkowski, P., Astot, C., Holub, J., Fuksová, K., Schmulling,<br />

T., Sandberg, G., Strnad, M. (2003): Identification of new aromatic cytokinins in<br />

Arabidopsis thaliana and Populus x canadiensis leaves by LC-(+)ESI-MS and capillary<br />

liquid chromato-graphy/frit-fast atom bombardment mass spectrometry. Physiol Plant<br />

117, 579-590<br />

/9/ Lebeda, A. (1986): Bremia lactucae. In: Lebeda, A. /ed./: Metody testování rezistence<br />

zelenin vůči rostlinným patogenům. VHJ Sempra, VŠÚZ Olomouc, 105-113<br />

/10/ Lichtenthaler, H.K. (1987): Chlorophylls and carotenoids – pigments of photosynthetic<br />

biomembranes. – In: Colowick, S.P., Kaplan, N.O. /ed./: Methods in Enzymology. Vol.<br />

148. Pp. 350-382. Academic Press, San Diego – New York – Berkeley – Boston –<br />

London – Sydney – Tokyo – Toronto<br />

/11/ Dan, K., Nagata, M., Yamashita, I. (1996): Changes in lipid peroxidation and antioxidant<br />

content in cotyledons of japanese radish. J Jap Soc Hort Sci 65, 603-608<br />

/12/ Sedlářová, M., Lebeda, A., Pink, D.A.C. (2001): The early stages of interaction between<br />

effective and non-effective race-specific genes in Lactuca <strong>sa</strong>tiva, wild Lactuca spp. and<br />

Bremia lactucae (race NL 16). J Plant Dis Prot 108, 477-489<br />

/13/ Vlčková, A., Špundová, M., Kotabová, E., Novotný, R., Doležal. K., Nauš, J. (2005):<br />

Protective cytokinin action switches to damaging during senescence of detached wheat<br />

leaves in continuous light. Physiol Plant, v recenzním řízení.<br />

/14/ Špundová M, Popelková H, Ilík P, Skotnica J, Novotný R, Nauš J (2003): Ultrastructural<br />

and functional changes in the chloroplasts of detached barley leaves senescing under dark<br />

and light conditions. J Plant Physiol 160, 1051–1058<br />

Effect of exogenous application of cytokinins on pathogenesis of Bremia<br />

lactucae and photosynthetic parameters of host plant<br />

Key words: Bremia lactucae – carotenoids – chlorophylls – Lactuca <strong>sa</strong>tiva –lipid<br />

peroxidation – photosystem II<br />

Summary<br />

Exogenous application of cytokinin solutions (2.10 -4 M meta-topolin and riboside 3-<br />

MeOBAPR) on detached leaf discs of susceptible plants Lactuca <strong>sa</strong>tiva L. (Cobham Green)<br />

24 h before inoculation suppressed sporulation and conidia formation of Bremia lactucae<br />

(cau<strong>sa</strong> agent of lettuce downy mildew). In host leaf tissue, the effect of cytokinin application<br />

on changes in photosynthetic parameters (chlorophyll and carotenoid content, photochemical<br />

efficiency of photosystem II) and cell membranes (level of lipid peroxidation) induced by<br />

senescence and pathogenesis was studied. The exogenous application of cytokinins did not<br />

influence the decrease in chlorophyll and carotenoid content induced by senescence.<br />

Cytokinins stimulated the decrease in the efficiency of PSII in the non-infected leaf discs,<br />

whereas in the infected ones they suppressed it. The exogenous application of cytokinins<br />

reduced the increase in lipid peroxidation in both infected and non-infected leaf discs.<br />

Poděkování<br />

Tyto výsledky jsou součástí projektu financovaného výzkumným záměrem MŠMT ČR č.<br />

MSM 6198959215 a GAČR 522/02/D011.<br />

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