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RATL: A Database of Reptile and Amphibian Toxicology Literature

RATL: A Database of Reptile and Amphibian Toxicology Literature

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Species<br />

Code b<br />

Lifestage Study<br />

Endpoint g<br />

pH Level(s)<br />

Temp h Additional<br />

Contam a<br />

RASY embryo HATSUC 4.2-7.5 15 or 3.5<br />

<strong>and</strong> 15.5<br />

Table 6 - pH Studies - 29<br />

Cont.<br />

Conc.<br />

Conc. e<br />

LC50<br />

e<br />

Units<br />

LC100 e<br />

Effects eg<br />

For embryos collected from bog site exposed<br />

to bog water (pH 4.2) only 11% normal<br />

hatch <strong>and</strong> 77% curl occurred; low<br />

temperature affected the hatching success <strong>of</strong><br />

RASY in bog water (100% mortality at early<br />

development).<br />

RASY embryo HATSUC 3.5-7.0 12, 21 0 % hatched at pH 3.0 at both 12 <strong>and</strong><br />

21°C.> 90 % hatched at pH 7.0. More<br />

hatched at higher temperature.<br />

RASY embryo HATSUC 4.2 bog, 7.5 fen 11 % hatch at pH 4.2 <strong>and</strong> 96.3 % hatch at<br />

pH 7.5; 77.2 % embryo developed by not<br />

hatched at pH 4.2.<br />

RASY embryo DEVOBS 4.0-5.8 80-100 % hatched at pH 5.0 <strong>and</strong> 5.8 with<br />

some deformities; at pH 4.5, 15 (at Ca 80<br />

ppm) - 100 % (Ca 20 ppm ) hatched . 0-13<br />

% hatched at pH 4.0.<br />

RASY embryo HATSUC 4.0-5.8 95-100 % hatching at pH 4.5 - 5.8; 80 %<br />

hatching at 4.25 (significantly greater than<br />

AMJE); 0 % hatching at pH 4.0.<br />

RASY embryo DEVOBS At pH 3.9, 50 % or more developed<br />

normally.<br />

RASY embryo BEHAV Breeding habitats in the Rocky Mountains do<br />

not appear to be sufficiently acidic to kill<br />

embryos.<br />

RASY embryo HATSUC 4.2, 4.4, 4.6,<br />

4.8, 6.0<br />

Al 0, 0.25,<br />

0.5, 0.75,<br />

1.0<br />

mg/L BUAM embryos were more sensitive to low<br />

pH than RASY embryos as indicated by a<br />

significant increase in mortality at pH 4.2<br />

<strong>and</strong> 0 mg/L Al/L. BUAM embryos were most<br />

stressed by Al at pH 4.2.<br />

Reference k<br />

Karns 1992<br />

Dale et al. 1985 k<br />

Karns 1984 k<br />

Freda <strong>and</strong> Dunson 1985 k<br />

Freda <strong>and</strong> Dunson 1986 k<br />

Gosner <strong>and</strong> Black 1957 k<br />

Corn <strong>and</strong> Vertucci 1992<br />

Freda <strong>and</strong> McDonald 1993<br />

RASY embryo BEHAV < 3.8-5.0 Al 114-834 Elevated Al <strong>and</strong> low pH may reduce breeding<br />

success.<br />

Glooschenko et al. 1986<br />

RASY embryo HATSUC 4.25 Hatched at 4.25.<br />

Freda <strong>and</strong> Dunson 1985 k<br />

RASY embryo HATSUC 2.0-7.6 Hatching success > 71 % at pH 4.0 - 7.6.<br />

Pierce et al. 1984 k<br />

RASY embryo HATSUC 3.5-6.3 20 3.5-4.0 Hatching success at pH 4.0 was significantly<br />

higher than at pH 3.5 but significantly lower<br />

than pH 4.5-6.3. No significant difference<br />

occurred between hatching success at pH 4.5<br />

or higher. The critical pH was determined to<br />

be pH 4.0-4.5.<br />

Grant <strong>and</strong> Licht 1993

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