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RATL: A Database of Reptile and Amphibian Toxicology Literature

RATL: A Database of Reptile and Amphibian Toxicology Literature

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Species<br />

Code b<br />

Lifestage Study<br />

Endpoint g<br />

pH Level(s)<br />

XELA embryo MORT 3.6-9.0 (third<br />

bioassay)<br />

Temp h Additional<br />

Contam a<br />

Table 6 - pH Studies - 39<br />

Cont.<br />

Conc.<br />

21-22 0.3-10 x<br />

normal<br />

Conc.<br />

e<br />

Units<br />

e<br />

LC50 LC100 e<br />

LC50 at<br />

pH 4.5 was<br />

2x<br />

blackwater<br />

XELA embryo MORT 3-10 21-22 Clearwater:<br />

3.92 (0.13<br />

SE)<br />

(jellied)<br />

3.93<br />

(0.081 SE)<br />

Clearwater:<br />

3.8<br />

(jellied),<br />

3.6<br />

(dejellied)<br />

Effects eg<br />

Dejellied embryos <strong>of</strong> XEGI <strong>and</strong> XELA<br />

showed comparable tolerances to increasing<br />

blackwater conc. No development occurred<br />

at pH 3.6. At pH 4.5 <strong>and</strong> above, survival<br />

increased <strong>and</strong> was only depressed in solutions<br />

<strong>of</strong> 2x, 5x <strong>and</strong> 10x blackwater.<br />

The pH at which survival was first noted<br />

differed with type <strong>of</strong> water, jellied embryos<br />

were more resistant to low pH stress in both<br />

tap (clearwater) <strong>and</strong> blackwater. Dejellied<br />

eggs <strong>of</strong> both species have similar tolerance<br />

levels.<br />

Reference k<br />

Picker et al. 1993<br />

Picker et al. 1993<br />

XELA embryo MORT 3-10 21-22 1x<br />

(dejellied)<br />

Blackwater Blackwater: The pH at which survival was first noted Picker et al. 1993<br />

normal<br />

: 5.03 4.2 (jellied) differed with type <strong>of</strong> water, jellied embryos<br />

(0.148 SE) 4.2 were more resistant to low pH stress in both<br />

(jellied) (dejellied) tap (clearwater) <strong>and</strong> blackwater. Dejellied<br />

4.51<br />

eggs <strong>of</strong> both species have similar tolerance<br />

(0.054 SE)<br />

(dejellied)<br />

levels.<br />

XELA embryo (<<br />

stage 4)<br />

HATSUC 3.5-6.0 21 Al 0.15-0.90 Results not extracted from paper.<br />

Dale et al. 1985 k<br />

XELA embryo<br />

(stages 10-<br />

DEVOBS 3.9-4.3 25 Tight coiling associated with shrinkage <strong>of</strong><br />

the perivitelline space; when jelly layer<br />

Dunson <strong>and</strong> Connell 1982<br />

13)<br />

removed at pH 4.3 the embryos developed<br />

normally.<br />

k<br />

XELA embryo- HATSUC 3.5-3.6 (Brook 20-24 36±14% developed into tadpoles. 57.2±22% Dumpert 1986<br />

metamorph<br />

I, II)<br />

<strong>of</strong> tadpoles were injured ie. bent backs,<br />

weaker pigmentation than controls. 40%<br />

tadpoles mortality occurred. Those that<br />

survived developed significantly slower than<br />

controls.<br />

XELA tadpoles DEVOBS 4.0, 7.2-7.6 25-30 One mortality occurred at neutral pH, 10 Pierce <strong>and</strong> Montgomery<br />

died at pH 4.0. Three d after exposure period 1989<br />

4 more tadpoles from the pH 4.0 group died.<br />

XXNE larvae BEHAV 4.5 <strong>and</strong> 7.4-7.5 20±3 TRCR showed no decline in the response to<br />

prey after transfer to pH 4.5. With only<br />

three exceptions, TRCR snapped <strong>and</strong><br />

consumed food immediately after it was<br />

<strong>of</strong>fered. All TRCR larvae achieved the<br />

maximum score <strong>of</strong> 5 in all tests.<br />

Griffiths et al. 1993

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