o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
3. ENVIRONMENTAL FATE AND PATHWAYS ID: 95-53-4
DATE: 07.01.2005
102
- The test substance concentration was monitored in regular intervals using
a scintillation counter.
Remark : 2-Nitrosotoluene, N-methyl-o-toluidine and N-formyl-o-toluidine were
detected as metabolites and these were considered as intermediates of the
detoxification pathway.
Result : The BCF calculated after 24 h of exposure was 4.6 +/- 0.8.
- Steady-state body burden was reached rapidly within 2-4 h of exposure
(uptake rate: 1.38+/-0.19 /h).
- Elimination: 85.1% (t1/2: 0.26-0.58 h).
- Residues of o-toluidine after 4 h were approximately equally distributed
within tissue compartments. After 24 h depuration the differences became
more pronounced.
- The distribution of o-toluidine in C. gigas tissues was: gills > viscera >
mantle > muscle.
Test condition : - Oysters (C. gigas) had an average shell length of 125.2 mm and an
average softbody wet weight of 11.7 g.
- The bivalves were kept in the laboratory in static aquaria containing
synthetic seawater kept at a constant temperature 15 °C, salinity= 30
promille and pH=7.9. The experiments were carried out under the same
conditions.
- The animals were not fed during the holding period and were utilised in
experiments within 3 weeks of their receipt.
Reliability : (2) valid with restrictions
Test procedure in accordance with generally accepted scientific standards
and described in sufficient detail
Flag : Critical study for SIDS endpoint
18.06.2004 (89)
Species : other: Mytilus edulis (Common bay mussel)
Exposure period : 24 hour(s) at 15 °C
Concentration : 5 mg/l
Elimination : yes
Method : other
Year : 1985
GLP : no data
Test substance : other TS: o-Toluidine, ring-labeled [14C], specific activity: 18.2 mCi/mM
(radiopurity > 99 %, tested by HPLC)
Method : - Static test with mussels from (1) pristine waters and (2) contaminated
waters.
- Single mussels exposed in 3 replicates.
- o-Toluidine loss was observed.
- BCF was determined at equilibrium.
- The test substance concentration was monitored in regular intervals using
a scintillation counter.
Remark : 2-nitrosotoluene, N-methyl-o-toluidine and N-formyl-o-toluidine as well as
N-hydroxy-o-toluidine (in smalller amount) were detected as metabolites
and these were considered as intermediates of the detoxification pathway.
Result : The BCF calculated after 24 h of exposure was 4.2 +/- 0.7.
- Steady-state body burden was reached rapidly within 4 h of exposure
(uptake rate: 0.82+/-0.38 /h).
- Elimination: 91.7% (t1/2: 0.36-0.52 h).
- The distribution of o-toluidine in M. edulis tissues was: gills > viscera >
mantle > muscle.
Test condition : - Mussels (M. edulis) had an average shell length of 68.5 mm and an
average softbody wet weight of 7.43 g.
- The bivalves were kept in the laboratory in static aquaria containing
synthetic seawater kept at a constant temperature 15 °C, salinity= 30
promille and pH=7.9. The experiments were carried out under the same
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