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o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

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OECD SIDS o-<strong>TOLUIDINE</strong><br />

4. ECOTOXICITY ID: <strong>95</strong>-<strong>53</strong>-4<br />

DATE: 07.01.2005<br />

118<br />

suspension was measured for each concentration of the test and control<br />

preparations.<br />

Remark : Microcystis aeruginosa is a blue-green algae (Cyanobacteria).<br />

Result : Reported TT (toxicity threshold) refers to nominal concentration and was<br />

determined at 3 % effect compared to the control (comparable to EC3)<br />

Test condition : - Test under static conditions<br />

- Nominal concentrations are given<br />

- Test temperature= 27 °C<br />

- Algal were submitted to continuous artificial light<br />

- pH=7<br />

- Turbidity of the solutions was measured.<br />

Reliability : (2) valid with restrictions<br />

Test procedure in accordance with generally accepted scientific standards<br />

and described in sufficient detail<br />

Flag : Critical study for SIDS endpoint<br />

13.07.2004 (109) (110)<br />

Species : Scenedesmus quadricauda (Algae)<br />

Endpoint : biomass<br />

Exposure period : 96 hour(s)<br />

Unit : mg/l<br />

EC3 : = 10<br />

Limit test :<br />

Analytical monitoring : no data<br />

Method : other: Cell multiplication inhibition test<br />

Year : 1<strong>95</strong>9<br />

GLP : no<br />

Test substance : other TS: o-Toluidine, purity is not specified<br />

Remark : Reported TT (toxicity threshold) refers to nominal concentration and is<br />

comparable to EC3<br />

Test condition : - The strain cultures were previously mixed with the following salts: 570<br />

mg/l KNO3, 200 mg/l CaSO4, 140 mg/l KH2PO4, 90 mg/lMgSO4, 3 mg/l<br />

FeCl3 and kept at day light and 24 °C. The culture medium was renewed<br />

every 2 months.<br />

Before the inoculation in the test system the strain culture is passed<br />

through a mebrane filter and then mixed with distilled water and again<br />

filtered.<br />

- Temperature= 24 °C<br />

- Continuous illumination<br />

Reliability : (2) valid with restrictions<br />

Basic data given<br />

18.06.2004 (105)<br />

Species : Scenedesmus quadricauda (Algae)<br />

Endpoint : biomass<br />

Exposure period : 8 day(s)<br />

Unit : mg/l<br />

EC3 : 6.3<br />

Limit test :<br />

Analytical monitoring : no data<br />

Method : other: cell multiplication inhibition test<br />

Year : 1977<br />

GLP : no<br />

Test substance : other TS: o-Toluidine, purity is not specified<br />

Method : Cell multiplication inhibition test according to Bringmann<br />

and Kuehn (1977)<br />

Remark : It is unclear whether the algae are within the exponential growth throughout<br />

the whole exposure period of 8 days.<br />

Result : Reported TT (Toxicity threshold) is comparable to EC3; value refers to<br />

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