o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
5. TOXICITY ID: 95-53-4
DATE: 07.01.2005
170
Test condition : METABOLIC ACTIVATION:
S9 mix liver homogenates from Aroclor 1254 induced rats
- a 3-hour treatment period and a 48 hour expression period were used
-cell colonies were counted 7-9 days after cells have been plated in soft
agar medium
-duplicate cultures were exposed to each concentration
- controls:
--negative control: solvent: DMSO
--positive control: 3-methylcholantrene and ethyl methane sulfonate
- percent total relative growth, an estimate of cytotoxicity and mutant
frequencies were calculated
-Evaluation of test results
a positive mutagenic response was indicated when 3 or more
concentrations satisfied all of the following:
--total relative growth: 20-80 %
--mutant frequencies have to be greater than the mutant frequencies for the
concurrent solvent control and grater than the range of acceptable mutant
frequencies for all solvent controls in this series
--absolute mean mutant yields significantly(p