o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
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OECD SIDS o-<strong>TOLUIDINE</strong><br />
5. TOXICITY ID: <strong>95</strong>-<strong>53</strong>-4<br />
DATE: 07.01.2005<br />
Hepatic DNA, RNA binding were determined according Cooper(1977), The<br />
tools of Biochemistry pp. 56 and Burton(1<strong>95</strong>6), Biochem. J. 62, 315<br />
Total protein binding was determined by method as described Hughes<br />
(1986) Carcinogenesis 7, 3-8; Protein was determined by the method of<br />
Lowery (1<strong>95</strong>1) J. Biol. Chem. 193, 265<br />
Reliability : (2) valid with restrictions<br />
study meets gtenerally acceptable principles<br />
13.05.2004 (131)<br />
In Vitro/in vivo : In vivo<br />
Type : Metabolism<br />
Species : rat<br />
Number of animals<br />
Males :<br />
Females :<br />
Doses<br />
Males : 0, 10, 100 mg/kg bw/day in corn oil<br />
Females :<br />
Vehicle : other: corn oil<br />
Route of administration : i.p.<br />
Exposure time : 7 day(s)<br />
Product type guidance :<br />
Decision on results on acute tox. tests :<br />
Adverse effects on prolonged exposure :<br />
Half-lives : 1 st :<br />
2 nd :<br />
3 rd :<br />
Toxic behaviour :<br />
Deg. product :<br />
Method : other: see freetext ME<br />
Year : 1988<br />
GLP : no data<br />
Test substance : other TS: o-toluidine, purity: 99 %<br />
Method : MaleSprague-Dawley rats were i.p. injected with 10 and 100 mg o-toluidine<br />
/kg bw/day dissolved in corn oil for 7 consecutive days . Rats were killed 24<br />
hours after the last injection.<br />
The study was carried out to examine the capacity to affect xenobiotic<br />
transformation in rats<br />
Result : 1) Dose dependant significant effects of o-toluidine on hepatic cytochrome<br />
P-450 monooxygenase activities and related enzymes (low dose, high<br />
dose versus control):<br />
Cytochrom P-450 (nmol/mg protein):<br />
0.88, 0.92 versus 0.68<br />
Ethoxyresorufin-O-deethylase (pmol/mg protein):<br />
103, 1100 versus 57<br />
Ethoxycoumarin-O-deethylase (pmol/mg protein):<br />
574, 1852 versus 420<br />
Aldrin epoxidase (nmol/min/mg protein):<br />
3.94, 4.05 versus 2.<strong>95</strong><br />
2) Significant effects of treatment by 100 mg o-toluidine on<br />
---activity of androstenedione hydoxylases (nmoles/min/mg protein):<br />
7-alpha-hydroxyandrost-4-ene-3,17-dione (7-alpha-OHA)<br />
6-beta-hydroxyandrost-4-ene-3,17-dione (6-beta-OHA)<br />
16-beta-hydroxyandrost-4-ene-3,17-dione (16-beta-OHA)<br />
16-alpha-hydroxyandrost-4-ene-3,17-dione (16-alpha-OHA)<br />
---and on testosterone formation (test-f) (nmoles/min/mg protein)<br />
treatment versus control:<br />
7-alpha-OHA: 0.18 versus 0.12<br />
<strong>UNEP</strong> PUBLICATIONS 135
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