o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
5. TOXICITY ID: 95-53-4
DATE: 07.01.2005
222
Test concentration : 0, 125, 250, 500, 1000 µg/ml
Cycotoxic concentr. : Preliminary cytotoxicity test
Metabolic activation : with and without
Result : positive
Method : other: see freetext TC
Year : 1985
GLP : no data
Test substance : other TS: o-toluidine, no data on purity
Result : Cytotoxicity was observed at the highest doses tested (no details)
o-Toluidine showed a statistically significant increase in SCEs in absence
of S9.
the positive controls were functional.
Test condition : Metabolic activation:
S9 mix was prepared from liver homogenates from Aroclor 1254 induced
male Sprague-Dawley rats
Solvent: DMSO
Controls:
negative control: solvent
positive controls: MNNG, BP, 2-AAF
Incubation time: 5 hours
statistic evaluation by student's t-test
Reliability : (2) valid with restrictions
no data on purity and no information about GLP
15.07.2004 (309)
Type : Sister chromatid exchange assay
System of testing : Lymphocytes (human)
Test concentration : 0, 1, 10, 100, 500, 600 µg/ml without S9-mix
0, 600 µg/ml with S9-mix
Cycotoxic concentr. : no data
Metabolic activation : with and without
Result : negative
Method : other: see freetext TC
Year : 1985
GLP : no data
Test substance : other TS: o-toluidine, no data on purity
Result : positive control were functional
without S9-mix: o-toluidine led to a slight elevation of the SCE rates, which
did not even reach a doublint of the control values.
Test condition : Method:
1-5 independent experiments with the blood from up to 5 different donors
were performed: pooled data were reported
Solvent: DMSO
Controls:
negative control: solvent
positive control: Diepoxybutane (DEB) in the absence of ---S9-mix;
Cyclophosphamid (CP) in the presence of S9-mix
Incubation time: 48 hours, without S9-mix; 47 hours, with ---S9-mix
METABOLIC ACTIVATION System:
S9 mix was prepared fron liver homogenates from Aroclor 1254 induced
male Sprague-Dawley rats
Reliability : (4) not assignable
only one dose used in the presence of metabolic activation, no data on
GLP and no data on purity of TS, no information about cytotoxicity
15.07.2004 (310)
Type : Sister chromatid exchange assay
System of testing : RL 4 cells (epithelial-like cells from rat liver)
Test concentration : (1) 0, 175, 350, 700 µg/ml (2) 21.8, 43.75, 87.5,µg/ml DMSO
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