o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

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OECD SIDS o-TOLUIDINE 4. ECOTOXICITY ID: 95-53-4 DATE: 07.01.2005 116 EC50 : 30.9 Limit test : no Analytical monitoring : yes Method : OECD Guide-line 201 "Algae, Growth Inhibition Test" Year : 1996 GLP : yes Test substance : other TS: 99 % purity Result : CONCENTRATIONS Recovery rates always > 88 % EFFECTS biomass: EbC50 (0-72h) = 30.9 mg/l (95 % confidence limits: 20.5-46.6 mg/l) NOECb (0-72h) = 2.91 mg/l growth rate: ErC50 (24-48h) > 150 mg/l NOECr (24-48h) = 2.91 mg/l ErC50 (24-72h) = 94.5 mg/l NOECr (24-72h) = 31 mg/l calculated: ErC50 (0-72h) = 110.9 mg/l MONITORING DATA water temperature: 22.8 - 23.3 °C pH: at 0h; 7.76 - 7.82, at 72h; 8.02 - 10.00 intensity of irradiation: 10,5000 lux (continuans) Test condition : TEST ORGANISMS strain: ATCC-22662 supplier: American Type Culture Collection pretreatment: 3 days CULTURE MEDIUM OECD medium TEST SYSTEM concentration (nominal): 0 (control), 1.23, 2.91, 6.40, 14.1, 31.0, 68.2, 150 mg/L renewal of test solution: none initial cell concentration: 1 x 10E+4 cells/mL exposure: 72 h, shaking (100 rpm) pH treatment: none exposure vessel: 300 mL net triangle conical glass flask number of replicate: 3 water temperature: 23 +- 2 °C temperature and light intensity were measured every 24 hours, whereas pH conditions at 0 and 72 hours photo period: continuous 15,000 lux test parameter: cells/mL Analytical monitoring: HPLC at 0 and 72 hours Reliability : (1) valid without restriction GLP guideline study Flag : Critical study for SIDS endpoint 07.01.2005 (107) Species : Chlorella pyrenoidosa (Algae) Endpoint : other: reduction of the maximum density (yield) Exposure period : 96 hour(s) Unit : mg/l EC50 : 55 Limit test : Analytical monitoring : no Method : OECD Guide-line 201 "Algae, Growth Inhibition Test" UNEP PUBLICATIONS
OECD SIDS o-TOLUIDINE 4. ECOTOXICITY ID: 95-53-4 DATE: 07.01.2005 Year : 1986 GLP : no data Test substance : other TS: o-Toluidine, purity > 99.5% Method : OECD TG 201 with slight modifications according to Van Leeuwen CJ et al. (1985). Aquatic toxicological aspects of dithiocarbamates and related compounds. Short-term tests. Aquat. Toxicol. 7, 145-164. Test condition : Stock solution of the test substance was prepared in dimethylsulfoxide (DMSO, Merck, Purity 99%) Reliability : (2) valid with restrictions Guideline study without detailed documentation Flag : Critical study for SIDS endpoint 10.11.2004 (30) Species : other algae: Scenedesmus obliquus Endpoint : growth rate Exposure period : 48 hour(s) Unit : mg/l EC50 : = 490 Limit test : Analytical monitoring : no data Method : OECD Guide-line 201 "Algae, Growth Inhibition Test" Year : 2001 GLP : no data Test substance : other TS: o-Toluidine, purity is not specified Result : Result was given as log (1/EC50)=2.34, where EC50 is given in mol/l. Furthermore the EC50 was calculated using QSAR-approach and a log (1/EC50)=2.58 for o-toluidine was obtained. This result corresponds to an EC50 of 282 mg/l. Test condition : - The algae in logarithmic growing period were inoculated into 250 ml flasks, amounting to 60 ml of culture media, compound and the algae. The initial algae cell concentration in the test culture was approximately 1E+04 cells/ml. - The culture was incubated under continuous light at a temperature of 20+/-1 °C for 48 h and the average illumination intensity was about 4000 lux produced by white fluorescent lamp. - Five concentration gradients were planned before beginning of the test. Space between gradients was 0.2. - Growth was monitored by electron microscope. Reliability : (2) valid with restrictions Guideline study without detailed documentation Flag : Critical study for SIDS endpoint 18.06.2004 (33) (108) Species : Microcystis aeruginosa (Algae, blue, cyanobacteria) Endpoint : biomass Exposure period : 8 day(s) Unit : mg/l EC3 : = .31 Limit test : Analytical monitoring : no Method : other: Cell multiplication inhibition test Year : 1978 GLP : no Test substance : other TS: o-Toluidine, purity is not specified Method : - Before starting the test, the stock solution of o-toluidine was neutralised. - The solutions with the test concentrations were then prepared in 300 ml Erlenmeyer flasks. - The extinction value of the monochromatic radiation (578 nm) of the cell UNEP PUBLICATIONS 117
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OECD SIDS o-TOLUIDINE FOREWORD INTR
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OECD SIDS o-TOLUIDINE 11. Comments:
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OECD SIDS o-TOLUIDINE and transitio
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OECD SIDS o-TOLUIDINE countries), e
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OECD SIDS o-TOLUIDINE 1.3 Physico-C
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OECD SIDS o-TOLUIDINE methylene-bis
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OECD SIDS o-TOLUIDINE Table 3 Photo
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OECD SIDS o-TOLUIDINE Inhalation Th
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OECD SIDS o-TOLUIDINE 6 REFERENCES
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OECD SIDS o-TOLUIDINE Bringmann G,
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OECD SIDS o-TOLUIDINE Hecht SS, El-
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OECD SIDS o-TOLUIDINE phase extract
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OECD SIDS o-TOLUIDINE Stark M (1892
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OECD SIDS o-TOLUIDINE I U C L I D D
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OECD SIDS o-TOLUIDINE 1. GENERAL IN
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o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

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