o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
5. TOXICITY ID: 95-53-4
DATE: 07.01.2005
228
No significant increase in MN frequency and no decrease in % PCEs was
observed at any sampling time. the concurrent positive controls were
functional.
(2) multiple-treatment study:
(2a) 1. trial
In a first multiple treatment study no decrease of % PCEs was observed,
but statistically significant increases of MNPCEs were induced at the high
dose (800 mg/kg) 24 h after the last of 2 and 4 treatments. Since the
mortality ratio after 4 injections was 3/6, this regimen was too toxic.
(2b) 2. trial
The experiment was repeated with similar results.
A weak MNPCE increase was observed only at highly toxic dose (800
mg/kg bw/day, 4 injections) which was statistically significant when
compared to the concurrent control:
800 mg/kg bw:
4 injections, 24 hours after the last injection:
mortality: 4/6
MNPCE/1000 PCEs: 2/6 mice: 0.4 % and 51.9 % PCE
1000 mg/kg bw:
2 injections, 24 hours asfter the last injection:
mortality: 6/6
negative control:
4 injections, 24 hours after the last injection:
MNPCEs/1000 PCEs: 6/6 mice: 0.12 % and 40.0 % PCE
historical control:
number of mice: 111
Number of PCEs observed: 111000
Mean of MNPCEs/1000 PCE: 1.12 %(ranged: 0-6)
Mean of % PCE: 46.5
The positive control was functional.
Test condition : TEST ORGANISMS:
- Age: 6 weeks
- No. of animals per dose: 6
ADMINISTRATION:
- Vehicle: olive oil
- Administration volume: 10 ml/kg body weight
- Duration of test:
-----------1. single-treatment study:
animals were killed 24, 48, 72 h after treatment with
dose levels: 0, 200, 400, 800 mg/kg b.w.
----------2. multiple-treatment study:
animals were killed 24h after 2 or 4 injections of o-toluidine at dose levels
of
a)0, 50 (4 inj.), 100, 200, 400, 800 mg/kg bw/day
b)0, 400, 600, 800,1000 mg/kg bw/day
- Sampling times and number of samples:
(1) 24, 48, 72 h for the single-treatment study
(2) 24 after last injection for the multiple-treatment
- 4 bone-marrow smears per animal
- Control groups:
negative controls: vehicle control group
positive controls: treated with single i.p.injection of 2 mg Mitomycin C/kg
bw; animals were killed 24h after
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