o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

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OECD SIDS o-TOLUIDINE 5. TOXICITY ID: 95-53-4 DATE: 07.01.2005 208 Test concentration : 0, 1, 10, 100, 1004 µg/ml in DMSO Cycotoxic concentr. : preliminary test up to 2000 µg/ml, >= 1004 µg/ml Metabolic activation : with and without Result : positive Method : other: according to Clive et al., 1979, Mutation Res. and Brusick, 1980 Year : 1985 GLP : no data Test substance : other TS: o-toluidine, no data on purity Result : o-toluidine was considered positive on the basis of a single strong result without S9 at the TK locus. Test condition : Preliminary cytotoxicity test Mutagenicity test Test-substance ability to induce mutation on two loki: (1) thymidin kinase and (2) Na+/K+ ATPase using multiwell plates. Exposure time: 2 hours, expression time: 48 hours 2 experiments with the selective agent ouabain (TK+/+) and 2 experiments with the selective agent trifluorothymidin (TK-/+) METABOLIC ACTIVATION: S9 mix prepared from liver homogenates from Aroclor 1254 induced rats Controls: negative controls = solvent (DMSO) positive controls = benzo[a]pyrene; ethyl methansulfonate Reliability : (2) valid with restrictions test system evaluation 15.07.2004 (173) Type : Mouse lymphoma assay System of testing : L5178Y TK+/TK+ Test concentration : range: 12.5 - 200 µl/ml in DMSO Cycotoxic concentr. : preliminary tests Metabolic activation : with and without Result : positive Method : other: according to Cole et al. 1979 Year : 1985 GLP : no data Test substance : other TS: o-toluidine, no data on purity Result : o-toluidine induced mutations to either ouabain or 6-thioguanine resistance. o-toluidene required liver S9 for mutation induction. Controls were functional Test condition : Mutation to ouabain and 6-thioguanine resistance was tested Controls: negative = solvent positive = benzo[a]pyrene; 4-nitroquinoline-1-oxide METABOLIC ACTIVATION: S9 mix liver homogenates from Aroclor 1254 induced rats Reliability : (2) valid with restrictions no detailed data on concentrations and results (only summery tables) 15.07.2004 (173) Type : Mouse lymphoma assay System of testing : L5178Y TK+/TK- Test concentration : no data Cycotoxic concentr. : no data Metabolic activation : with and without Result : negative UNEP PUBLICATIONS
OECD SIDS o-TOLUIDINE 5. TOXICITY ID: 95-53-4 DATE: 07.01.2005 Method : other: as described by Clive and Spector (1975): Mutat. Res. 31, 17-29 Year : 1985 GLP : no data Test substance : other TS: o-toluidine, no data on purity Test condition : METABOLIC ACTIVATION: S9 mix liver homogenates from Aroclor 1254 induced rats Reliability : (4) not assignable assay system evaluation, detailed data such as concentration range cytotoxicity and results in detail are not reported 15.07.2004 (173) (273) Type : Cytogenetic assay System of testing : Chinese hamster primary liver (CH1-L) cells Test concentration : 12, 30, 60, 120 ug/ml Cycotoxic concentr. : priliminary cytotoxicity test Metabolic activation : no data Result : negative Method : other: differential staining technique described by Wissinger et al. (1981) Year : 1985 GLP : no data Test substance : other TS: o-toluidine, no data on purity Result : A small increase in the mitotic index was seen with treatment. The AT/M ratio remained unaffected as did the numbers of abnormal division stages. There was no effect on either chromosome dislocation, multipolar spindels or anaphase lagging and bridges. Thus this agent did not affect the spindle. Test condition : The experiments were performed in parallel with those described by Danford N (1985) Prog Mutat Res 5: 397-411 measuring aneuploidy and chromosomal aberrations. The same cultures and test agent were used in these two studies. A differential staining technique described by Wissinger et al. (1981) that allows for the simultaneous visualisation of chromosomes and spindle fibres has been used to investigate the effects on cell division and spindle formation. Reliability : (4) not assignable special study 15.07.2004 (278) (249) Type : other: (1) Alkaline sucrose gradient (ASG) - , (2) Micronucleus - and (3) SCE-assay System of testing : Chinese hamster ovary (CHO) cells Test concentration : (1) 3210-7500 µg/ml in DMSO (2) no data (3) 0, 1.07-1070 µg/ml in DMSO Cycotoxic concentr. : 7500 µg/ml Metabolic activation : with and without Result : Method : other: see freetext ME Year : 1985 GLP : no data Test substance : other TS: o-toluidine, no data on purity Method : Short-term tests in Chinese-hamster ovary cells: ----(1) Induction of alkaline sucrose gradient (ASG) as assay to measure DNA single-strand breaks - -detectable DNA damage (Douglas and Grant, 1980, Mutation Res. 77 259-269) ----(2) Micronucleous assay for measuring chromosome damage (Heddle and Carrano, 1977, Mutation Res. 44, 63-69) ----(3) Sister-chromatid exchange (Perry and Evans, 1975, Nature(London) 258, 121-125). UNEP PUBLICATIONS 209
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OECD SIDS o-TOLUIDINE FOREWORD INTR
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OECD SIDS o-TOLUIDINE 11. Comments:
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OECD SIDS o-TOLUIDINE and transitio
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OECD SIDS o-TOLUIDINE 6 REFERENCES
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OECD SIDS o-TOLUIDINE Bringmann G,
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OECD SIDS o-TOLUIDINE Hecht SS, El-
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OECD SIDS o-TOLUIDINE Stark M (1892
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OECD SIDS o-TOLUIDINE I U C L I D D
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o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

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