o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
5. TOXICITY ID: 95-53-4
DATE: 07.01.2005
166
Result : The positive controls were functional.
Reliability : (1) valid without restriction
equivalent ot OECD guideline
Flag : Critical study for SIDS endpoint
02.06.2004 (168)
Type : Ames test
System of testing : Salmonella typhimurium TA 97, TA 98, TA 100, TA 102
Test concentration : 0, 100, 200, 500, 1000, 2000, 5000 µg/plate in DMSO
Cycotoxic concentr. : 5000 µg/plate
Metabolic activation : with and without
Result : negative
Method : other: according Matsushima et al. (1980) in: Short term test systems for
detecting carc.Norporth K, Springer, New York: preincubation method
(30°C, 30 min)
Year : 1980
GLP : no data
Test substance : other TS: o-toluidine, no data on purity
Result : No dose-related realted increase in mutant frequency were shown. The
number of revertant colonies per plate with the test substance was not
more than twice of that of the negative control.
Test condition : Tests were carried out by preincubation method (30 min) with and without a
metabolic activation system.
METABOLIC ACTIVATION:
S9 mix liver homogenates from phenobarbital and 5,6-benzoflavone
induced male Sprague-Dawley rats
Positive controls:
with S9: 2-aminoanthracene; benzo[a]pyrene
without S9: 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide; bleomycin
The positive controls increased mutant counts to well over those of the
negative controls, and thus demonstrated the system's sensitivity and the
activity of the S9 mix.
Criteria for positive results not given.
Reliability : (2) valid with restrictions
only 4 strains used
Flag : Critical study for SIDS endpoint
21.05.2004 (169)
Type : Ames test
System of testing : Salmonella typhimurium TA 98, TA 100, TA 1535, TA 1537, TA 1538
Test concentration : 0, 50, 100, 500, 1000, 5000 µg/plate in DMSO
Cycotoxic concentr. : > 5000 µg/plate
Metabolic activation : with and without
Result : negative
Method : other: according to Ames et al., Mutat. Res. 31, 347 (1975)
Year : 1985
GLP : no data
Test substance : other TS: o-toluidine, no data on purity
Result : Treatment with o-toluidine resulted in neither toxicity nor the induction of
point mutation in Salmonella either with or without metabolic activation.
Test condition : TEST METHODOLOGY:
plate-incorporation assay
METABOLIC ACTIVATION:
S9 mix liver homogenates from Aroclor 1254 induced rats
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