o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
5. TOXICITY ID: 95-53-4
DATE: 07.01.2005
214
o-toluidine was tested positive in the presence of S9-mix although no dose
response relationship was noted.
The positive controls were reported to be fucntional.
Test condition : This study is part of a International Collaborative Program, published as:
Evaluation of Short-Term Tests for Cancerogens Progress in Mutation
Research Vol 1. Edited by Frederick J. Serres and John Ashby, 1981
CHEMICALS:
42 coded chemicals were investigated in short-term tests for
cancerogenicity in different laboratories
It should be suggested that only 100%-pure chemicals should be used
when evaluating a test system.
---In the present work the compound with code no 41 is declared as otoluidine
hydrochloride, whereas in an the overall summary of this program
code no. 41 is declared as o-toluidine.
METABOLIC ACTIVATION:
S9 mix - liver homogenates from with phenobarbitone induced male Wistar
rats
Controls:
negative control: solvent (DMSO)
positive controls:
in the presence of S9-mix: cyclophosphamide
in the absence of S9-mix: 4-nitroquinoline-1-oxide
Incubation time: 2.5 hours
Stastistical analysis: student t'Test
Reliability : (4) not assignable
Method-Evaluation, no data on test concentration, no data on cytotoxicity,
unclear use: o-toluidine or o-toluidine hydrochloride
15.07.2004 (152) (290)
Type : Unscheduled DNA synthesis
System of testing : primary rat hepatocytes
Test concentration : 0.02 - 0.000001 M in DMSO
Cycotoxic concentr. : no data
Metabolic activation : without
Result : positive
Method : other: according Althaus et al., 1982, Cancer Res. 42, 3010
Year : 1985
GLP : no data
Test substance : other TS: o-toluidine, no data on purity
Result : significant increase; 176% of control induced UDS at the optimal
concentration
Test condition : Incubation time: 18 hours
Nuclear isolation followed by scintillation counting of extracted DNA
Metabolic activation: intrinsic
Controls: UV
Doses tested 0.02 - 0.00002 M for dose-response curve,
max effective dose for further testing 0.002 M
Definition of positive results:
Means at maximum effective dose compared by a 1-sided Student`s t-test
or Lohrding`s test. A sample mean which was greater than a control mean
with > 95% confidence was considered positive
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