o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
5. TOXICITY ID: 95-53-4
DATE: 07.01.2005
164
CRITERIA FOR POSITIVE RESULT:
in general:
dose-related increase in the mean revertants per plate of at least one tester
strain with a minimum of two increasing concentrations of test article
S.typh.TA1535 and TA1537:
increase in mean revertants at the peak of the dose response is equal or
greater than three times the mean vehicle control value S.typh.TA98 and
TA100:
increase in mean revertants at the peak of the dose response is equal or
greater than two times the mean vehicle control value
Result : 1) o-toluidine was negative in all tester strains without or with metabolic
activation
Positive controls were functional
2) o-toluidine did cause positive responses with strain TA98 in the
presence of aroclor-induced rat liver-S9 and Norharman.
Reliability : (2) valid with restrictions
only 4 strains used
Flag : Critical study for SIDS endpoint
24.05.2004 (164)
Type : Ames test
System of testing : Salmonella typhimurium TA 97, TA 98, TA 100, TA 1535
Test concentration : 0, 33, 100, 333, 500, 1000, 2000, 3333 µg/plate in DMSO
Cycotoxic concentr. : > 3333 µg/plate
Metabolic activation : with and without
Result : negative
Method : other: preincubation method according to Ames, Mutat. Res. 31,347(1975);
Maron, Mutat. Res.113,173(1983), see also freetext TC
Year : 1985
GLP : no data
Test substance : other TS: o-toluidine, no data on purity
Result : o-Toluidine was without significant activity without S9-mix
with S9 mix of rats and hamsters with one exception:
o-Toluidine induced a dose-related increase of revertants in TA100 only in
the presence of 30% hamster liver S9-mix
Test condition : Salmonella strains were used in a 20 min preincubation procedure without
metabolic activation and with Aroclor-1554-induced S9 from male Sprague-
Dawley rats(10%, 30%) and Syrian hamsters (10%, 30%).
Dosing:
Chemicals were tested under code at a minimum of five doses up to a toxic
dose or the limit of solubility, to a maximum dose of 10 mg per plate.
Evaluation:
A positive response was defined as a dose-related increase over the
controlregardless of its magnitude.
In the first experiment the concentration of S9-mix was 10%.
If all tests (non activation, rat S9 and hamster S9 were negative, they were
repeated, with 30% S9-mix.
If the repeat test was also negative, the chemical was declared
nonmutagenic.
If a positive result was seen, only the strain-activation combination giving
the positive result, was repeated.
If an equivocal or weak response was seen, only that strain-activation
combination was repeated;
Concurrent positive and negative controls were run.
negative control: solvent = DMSO
positive controls:
without S9-mix:
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