o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

More documents

Recommendations

Info

OECD SIDS o-TOLUIDINE 5. TOXICITY ID: 95-53-4 DATE: 07.01.2005 206 The positive controls were functional. Test condition : Treatment Time: without S9-mix: 48 hours with S9-mix: 3 hours Controls: negative control: solvent (DMSO) positive control: Dimethylnitrosamin (DMN), Benzopyrene (BP) METABOLIC ACTIVATION: S9 mix liver homogenates with phenobarbital and 5,6-benzoflavone induced rats Reliability : (2) valid with restrictions assay system evaluation 15.07.2004 (173) (274) Type : Mammalian cell gene mutation assay System of testing : human cell lines:TK6-cells ((thymine kinase locus) and AHH-1-cells (Hypoxanthine guanine phosphoribosyl transferase locus) Test concentration : Tk6-cells: -S9-mix: 0, 150, 300, 450, 600, 750 µg/ml; +S9-mix: 0, 450, 900, 1350 µg/ml AHH-1-cells:-S9-mix: 0, 100, 200, 300, 400, 500 µg/ml; Cycotoxic concentr. : preliminary cytotoxicity test Metabolic activation : with and without Result : positive Method : other: human lymphoblast gene-locus mutation assay, according Thilly et al. (1976), Chem.-Biol. Interact 15, 33-50 Year : 1985 GLP : no data Test substance : other TS: o-toluidine, no data on purity Method : Preliminary cytotoxicity determination to define the dose-range Mutation test: negative control was the solvent: DMSO positive controls: 4-nitroquinoline-N-oxide, benzo(a)pyrene Exposure time: TK6-cells, -S9-mix: 20 hours (approx. one cell generation) TK6-cells, +S9-mix: 3 hours AHH-1-cells (metabolic competent): 28 hours (approx. one cell generation) Metabolic activation system: Arocolor 1254 activated rat liver cells Result : o-toluidine was mutagenic in TK6 cells in the presece of S9-mix at 450 µg/ml and higher and in the metabolic competent AHH-1 cells at 300 µg/ml and higher. The positive controls were functional. Reliability : (2) valid with restrictions assay system evaluation, well documented 15.07.2004 (173) Type : Mouse lymphoma assay System of testing : L5178Y TK-/TK+ Test concentration : 0, 0.1, 0.3, 1.0, 3.0, 10 µl/ml Cycotoxic concentr. : 1 µl/ml = ca. 1mg/µl Metabolic activation : with and without Result : negative Method : other Year : 1985 GLP : no data Test substance : other TS: o-toluidine, no data on purity Result : o-toluidine at a concentration of 2µl/ml or higher for 2h was almost completely toxic to cells. No mutagenic effect was observed under any of the test conditions. UNEP PUBLICATIONS
OECD SIDS o-TOLUIDINE 5. TOXICITY ID: 95-53-4 DATE: 07.01.2005 Test condition : METABOLIC ACTIVATION: Several systems were applied: S9 mix liver homogenates from Aroclor 1254 induced rats primary rat hepatocytes, primary chick-embryo hepatocytes and Syrian hamster-embryo cells Reliability : (2) valid with restrictions assay system evaluation 15.07.2004 (173) (275) Type : Mouse lymphoma assay System of testing : L15178Y TK-/TK+ Test concentration : 1) 0, 125, 250, 375, 500, 625, 750, 875, 1000 µg/ml without activation 2) 0, 10, 25, 50, 75, 100, 200, 300, 400 µg/ml with metabolic activation Cycotoxic concentr. : preliminary toxicity test for dose range finding Metabolic activation : with and without Result : negative Method : other: according Clive et al., 1979, Mutation Res. 59, 61 Year : 1983 GLP : no data Test substance : other TS: o-toluidine, no data on purity Remark : positive controls were functional Test condition : Controls: negative control = solvent (DMSO positive controls: ethyl methanesulfonate without S9 and 3methylcholanthrene with S9 METABOLIC ACTIVATION: S9 mix was prepared from liver homogenates from Aroclor 1254 induced rats Reliability : (2) valid with restrictions assay system evaluation 15.07.2004 (173) (276) Type : Mouse lymphoma assay System of testing : L15178Y TK-/TK+ Test concentration : -S9-mix: 0, 600, 700, 800, 900, 1000, 1100, 1200, 1300 µg/ml; +S9-mix: 0, 125, 200, 275, 350, 425, 500, 575, 650 µg/ml Cycotoxic concentr. : -S9-mix: >= 600 µg/ml; +S9-mix: >= 350 µg/ml Metabolic activation : with and without Result : negative Method : other: according Oberly et al., 1982, J.Toxicol. Environm. Health 9, 367 Year : 1983 GLP : no data Test substance : other TS: o-toluidine, pure techn. graded Test condition : CONTROLS: negative control = solvent (DMSO) positive controls: ethyl methanesulfonate without S9 and 3methylcholanthrene with S9 METABOLIC ACTIVATION: S9 mix was prepared from liver homogenates from Aroclor 1254 induced rats Reliability : (2) valid with restrictions Method evaluation 15.07.2004 (277) Type : Mouse lymphoma assay System of testing : L5178Y TK-/TK+ and TK+/TK+ UNEP PUBLICATIONS 207
Page 1 and 2:
OECD SIDS o-TOLUIDINE FOREWORD INTR
Page 3 and 4:
OECD SIDS o-TOLUIDINE 11. Comments:
Page 5 and 6:
OECD SIDS o-TOLUIDINE and transitio
Page 7 and 8:
OECD SIDS o-TOLUIDINE countries), e
Page 9 and 10:
OECD SIDS o-TOLUIDINE 1.3 Physico-C
Page 11 and 12:
OECD SIDS o-TOLUIDINE methylene-bis
Page 13 and 14:
OECD SIDS o-TOLUIDINE Table 3 Photo
Page 15 and 16:
OECD SIDS o-TOLUIDINE With adapted
Page 17 and 18:
OECD SIDS o-TOLUIDINE Table 8 Geoac
Page 19 and 20:
OECD SIDS o-TOLUIDINE polluted site
Page 21 and 22:
OECD SIDS o-TOLUIDINE depending on
Page 23 and 24:
OECD SIDS o-TOLUIDINE prepared from
Page 25 and 26:
OECD SIDS o-TOLUIDINE 3.1.2 Acute T
Page 27 and 28:
OECD SIDS o-TOLUIDINE 3.1.4 Sensiti
Page 29 and 30:
OECD SIDS o-TOLUIDINE Inhalation Th
Page 31 and 32:
OECD SIDS o-TOLUIDINE males or fema
Page 33 and 34:
OECD SIDS o-TOLUIDINE examination y
Page 35 and 36:
OECD SIDS o-TOLUIDINE Conclusion Th
Page 37 and 38:
OECD SIDS o-TOLUIDINE According to
Page 39 and 40:
OECD SIDS o-TOLUIDINE The lowest te
Page 41 and 42:
OECD SIDS o-TOLUIDINE 4.2 Terrestri
Page 43 and 44:
OECD SIDS o-TOLUIDINE 6 REFERENCES
Page 45 and 46:
OECD SIDS o-TOLUIDINE Bringmann G,
Page 47 and 48:
OECD SIDS o-TOLUIDINE Hecht SS, El-
Page 49 and 50:
OECD SIDS o-TOLUIDINE phase extract
Page 51 and 52:
OECD SIDS o-TOLUIDINE Stark M (1892
Page 53 and 54:
OECD SIDS o-TOLUIDINE I U C L I D D
Page 55 and 56:
OECD SIDS o-TOLUIDINE 1. GENERAL IN
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
OECD SIDS o-TOLUIDINE 2. PHYSICO-CH
Page 63 and 64:
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
OECD SIDS o-TOLUIDINE 3. ENVIRONMEN
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
Page 91 and 92:
Page 93 and 94:
Page 95 and 96:
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
OECD SIDS o-TOLUIDINE 4. ECOTOXICIT
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
OECD SIDS o-TOLUIDINE 5. TOXICITY I
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156: OECD SIDS o-TOLUIDINE 5. TOXICITY I
Page 205: OECD SIDS o-TOLUIDINE 5. TOXICITY I
Page 257 and 258:
Page 259 and 260:
Page 261 and 262:
Page 263 and 264:
Page 265 and 266:
Page 267 and 268:
Page 269 and 270:
Page 271 and 272:
Page 273 and 274:
Page 275 and 276:
OECD SIDS o-TOLUIDINE 6. REFERENCES
Page 277 and 278:
Page 279 and 280:
Page 281 and 282:
Page 283 and 284:
Page 285 and 286:
Page 287 and 288:
Page 289 and 290:
Page 291 and 292:
Page 293 and 294:
Page 295 and 296:
Page 297:
show all

o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

Create successful ePaper yourself

Delete template?

Save as template?